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Virus Detection and Innate Immune Activation

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Detection of Cytoplasmic Viral Nucleic Acid and Cell Intrinsic Innate Immune Activation

• ADAR1
• ATF-4
• ATF-6
• BiP
• CHOP
• DDX41
• eIF2α
• Phospho-eIF2α (Ser51)
• cGAS
• GCN2
• IFN-α
• IFN-β1
• IκBα
• Phospho-IκBα (Ser32/36)
• IKKα
• Phospho-IKKα/β (Ser176/180)
• Phospho-IKKβ (Ser177/181)
• IKKε
• Phospho-IKKε (Ser172)
• IKKγ
• IRE1α
• IRF-3
• Phospho-IRF-3 (Ser379)
• Phospho-IRF-3 (Ser386)
• Phospho-IRF-3 (Ser396)
• IRF-7
• Phospho-IRF-7 (Ser437/438)
• Phospho-IRF-7 (Ser471/472)
• Phospho-IRF-7 (Ser477/Ser479)
• LGP2
• MAVS
• MDA5
• NF-κB p65
• Phospho-NF-κB p65 (Ser468)
• Phospho-NF-κB p65 (Ser536)
• NLRX1
• OAS1
• PERK
• Phospho-PERK (Thr980)
• PKR
• c-Rel
• RelB
• RIG-I
• RNase L
• SINTBAD
• STING
• Phospho-STING (Ser365) mouse
• Phospho-STING (Ser366)
• TAK1
• Phospho-TAK1 (Thr184/187)
• TBK1/NAK
• Phospho-TBK1/NAK (Ser172)
• TRAF3
• TRAF6
• TREX1
• XBP-1s
• ZBP1
• ZCCHC3

TLRs and Innate Immune Activation

• IFN-α
• IFN-β1
• IκBα
• Phospho-IκBα (Ser32/36)
• IKKα
• Phospho-IKKα/β (Ser176/180)
• Phospho-IKKβ (Ser177/181)
• IKKε
• Phospho-IKKε (Ser172)
• IKKγ
• IRAK1
• IRAK2
• IRAK3
• IRAK4
• Phospho-IRAK4 (Thr345/Ser346)
• IRF-3
• Phospho-IRF-3 (Ser379)
• Phospho-IRF-3 (Ser386)
• Phospho-IRF-3 (Ser396)
• IRF-5
• IRF-7
• Phospho-IRF-7 (Ser437/438)
• Phospho-IRF-7 (Ser471/472)
• Phospho-IRF-7 (Ser477/Ser479)
• Myeloperoxidase
• MyD88
• NF-κB p65
• Phospho-NF-κB p65 (Ser468)
• Phospho-NF-κB p65 (Ser536)
• Pellino-1
• c-Rel
• RelB
• SARM1
• TAK1
• Phospho-TAK1 (Thr184/187)
• TBK1/NAK
• Phospho-TBK1/NAK (Ser172)
• TIRAP
• Tollip
• Toll-like Receptor 1
• Toll-like Receptor 2
• Toll-like Receptor 3
• Toll-like Receptor 6
• Toll-like Receptor 7
• Toll-like Receptor 8
• Toll-like Receptor 9
• TRAF3
• TRAF6
• TRIF

Inflammasome Activation

• ASC/TMS1
• Caspase-1
• Cleaved Caspase-1 (Asp296) mouse
• Cleaved Caspase-1 (Asp297)
• Caspase-11
• Gasdermin D
• Cleaved Gasdermin D (Asp276) mouse
• Cleaved Gasdermin D (Asp275)
• Cleaved-IL-1β (Asp116)
• Cleaved-IL-1β (Asp117) mouse
• NLRP3

Markers of Innate Immune Cells

• CCR2
• CD11b/ITGAM
• CD11c
• CD14
• CD16
• CD47
• CD68
• CD80
• CD83
• CD86
• CD161/KLRB1
• M-CSF Receptor
• F4/80
• HLA-DRA
• IRF-8
• Ly-6G
• NCAM1
• Neutrophil Elastase
• XCR1

Related Kits & Reagents

• ER Stress Antibody Sampler Kit

• Mouse Reactive Inflammasome Antibody Sampler Kit

• Innate Immunity Activation Antibody Sampler Kit

• Human-Reactive STING Pathway Antibody Sampler Kit

• Mouse-Reactive STING Pathway Antibody Sampler Kit

• NF-κB Pathway Sampler Kit

• NF-κB p65 Antibody Sampler Kit

• PhosphoPlus^® PERK (Thr980) Antibody Duet

• Rig-I Pathway Antibody Sampler Kit

• Toll-like Receptor Antibody Sampler Kit

• Toll-like Receptor Antibody Sampler Kit II

• Thapsigargin

• Tunicamycin

Resources

• Cell Intrinsic Innate Immunity Signaling

• Immune Cell Marker Guide for Flow Cytometry (Human)

• Immune Cell Marker Guide for Flow Cytometry (Mouse)

• Toll-like Receptor Signaling

• Blog: How does the innate immune system work?

• Protocol Compatibility Table for Immune Signaling and Phenotyping by Flow Cytometry