CUT&Tag Resource Center

CUT&Tag: An Introduction

Cleavage Under Targets & Tagmentation (CUT&Tag), is a low-cell number chromatin profiling method, similar to CUT&RUN. It differs from CUT&RUN by employing a primary antibody, a secondary antibody, and pAG-Tn5 to identify protein-DNA interactions across the genome for analysis with next-generation sequencing (NGS). A key advantage of CUT&Tag is that the Tn5 also completes the adaptor ligation as part of the assay, resulting in a faster, streamlined DNA library prep. CUT&Tag is also compatible with the use of Drosophila normalization spike-in controls, which correct for technical variability between samples for the entire experimental workflow—from cells to sequenced DNA. We have compiled a collection of videos, application notes, eBooks, blogs, and more to help you start using CUT&Tag to profile histones, transcription factors, and cofactors. Go from cells to DNA library in just 1-2 days.

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