G4S Linker (E7O2V) Rabbit Monoclonal Antibody (PE Conjugate) (Translational-Grade) #99539
- F
Product Specifications
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
The product is tested by flow cytometry using human T cells expressing scFv-based CARs containing a G4S linker.
The product can be used for research or manufacturing of cell-, gene-, or tissue-based products. Not intended for diagnostic use or direct administration into humans or animals.
Technical Specifications
Activity (Flow Cytometry): ≥ 80% relative MFI using a stable anti-CD19 CAR Jurkat cell line compared to a reference standard
Non-Specific Binding (Flow Cyometry): ≤ 2% positive events when tested on CD3+ primary human T cells, relative to a concentration-matched isotype control
Endotoxin: (USP<85>, Ph. Eur. 2.6.14): ≤ 4.00 EU/mL
Residual Protein A (ELISA)1: Available upon request
Residual Host Cell Protein (ELISA)2: ≤ 100 ng/mg
Residual Host Cell DNA (qPCR)2,4: < 0.5 pg/mg
Mycoplasma: (USP<63>, Ph. Eur. 2.6.7)3: Negative
1Residual Protein A testing is completed on the bulk antibody-conjugate, G4S Linker (E7O2V) Rabbit Monoclonal Antibody (PE Conjugate) (GMP-Grade). Results are available upon request.
2Residual Host Cell Protein and Residual Host Cell DNA testing is completed on pre-conjugated G4S Linker (E7O2V) Rabbit Monoclonal Antibody (GMP-Grade).
3Mycoplasma is tested on unprocessed bulk, pre-conjugated G4S Linker (E7O2V) Rabbit Monoclonal Antibody (GMP-Grade).
4Value is the actual result from pre-conjugated G4S Linker (E7O2V) Rabbit Monoclonal Antibody (GMP-Grade) testing.
Fluorescent Properties
- ← Excitation: 565 nm ← Emission: 575 nm
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
Limited Uses
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