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Kinase Buffer (10X) #9802

    Product Information

    Product Usage Information

    1. Thaw 10X buffer at 24-30°C, mixing end-over-end.
    2. Dilute the 10X buffer to the desired concentration using ddH2O.

    Storage

    1X Kinase Buffer:

    25 mM Tris-HCl (pH 7.5), 5 mM beta-glycerophosphate, 2 mM dithiothreitol (DTT), 0.1 mM Na3VO4, 10 mM MgCl2.

    Note: Kinase Buffer must be supplemented with ATP (#9804).

    For long term storage, keep 10X buffer at -20°C If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. Aliquoting of 10X buffer is recommended if many small experiments are to be performed. 1X solution can also be aliquotted into single use aliquots and stored at -20°C.

    Product Description

    Kinase Buffer can be used to assay protein kinase activity.
    For Research Use Only. Not for Use in Diagnostic Procedures.
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