PathScan® Sandwich ELISA Lysis Buffer (1X) #7018
Product Information
Product Usage Information
1. Thaw 10x buffer at 24-30°C, mixing end-over-end.
2. Dilute 10X ELISA Lysis Buffer to a 1X solution using ddH2O.
3. Chill 1X buffer on ice and add 1mM PMSF just prior to use.
Lysis:
All reagents and lysates must be kept cold.
1. Treat cells as desired.
2. Wash plate with PBS to remove residual media.
3. Add 1X to cells:
4. Incubate plate on ice for 2 minutes.
5. Collect cell lysate solution for use.
6. Centrifuge extract for 5 minutes at 14,000 x g in a cold microfuge.
7. Remove supernatant for use.
Additional protease inhibitors can be added to the 1X lysis buffer without any difficulties.
Storage
20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% Triton, 20 mM sodium Pyrophosphate, 25 mM Sodium Fluoride, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin
For long term storage, keep 10X buffer at -20°C If buffer will be continually used, it is recommended that the 10X buffer be kept at 4°C for 1-2 weeks. Aliquoting of 10X buffer is recommended if many small experiments are to be performed. 1X solution can also be aliquotted into single use aliquots and stored at -20°C.
Product Description
Background
Limited Uses
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