PTMScan® Anti-Biotin Kit #41343
Additional Information
This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.
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Product Description
PTMScan® technology employs a proprietary methodology from Cell Signaling Technology (CST) for post-translationally modified (PTM) peptide enrichment by immunoprecipitation using a specific agarose bead-conjugated antibody in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for quantitative profiling of PTM sites in cellular proteins. PTMs analyzed using this technology include phosphorylation, ubiquitination, acetylation, and methylation, among others. The methodology enables researchers to isolate, identify, and quantify large numbers of PTM cellular peptides. The workflow provides a global, unbiased overview of PTMs across cell and tissue samples of any species, regardless of modification site location. For more information on PTMScan products and services, please visit the Proteomics Resource Center.
Background
New assays have been developed that improve elucidation of protein subcellular context, localization, and protein-protein interactions. These new cellular assays utilize biotin ligase or engineered ascorbate peroxidase (APEX) fusion proteins that are able to biotinylate nearby protein targets within specific cellular compartments. This type of biotin proximity assay can provide improved information about subcellular localization, multi-subunit components, and recruitment of unknown targets. Using CST's in house developed anti-biotin rabbit monoclonal antibody; we have developed a robust procedure for immunoaffinity enrichment followed by mass spectrometry analysis using CST’s proprietary PTMScan(R) protocols. This allows for identification of biotinylated targets and investigation of new protein interactions. This assay provides rich data for these types of biotin proximity assays such as the APEX (6,7) engineered cell system and other related methods.
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- Wright, L.D. et al. (1947) Proc Soc Exp Biol Med 64, 150-3.
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- Hofmann, K. and Kiso, Y. (1976) Proc Natl Acad Sci U S A 73, 3516-8.
- Shamsuddin, A.M. and Harris, C.C. (1983) Arch Pathol Lab Med 107, 514-7.
- Paek, J. et al. (2017) Cell 169, 338-349.e11.
- Hung, V. et al. (2016) Nat Protoc 11, 456-75.
Alternate Names
apex; biotin; motif
Limited Uses
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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.