Render Target: SSR
Render Timestamp:
9/21/2026, 9:05:29 AM EDT
9/21/2026, 1:05:29 PM UTC
Commit: 916faa3125cd7e5a4910ff1f822b1dee8ea9bfe0
XML generation date: 2026-07-20 16:21:17.145
Product last modified at: 2026-07-21T07:00:59.251Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Phospho-Erk5 (Thr218/Tyr220) Antibody #3371

Filter:
  • WB

    Product Specifications

    REACTIVITY H
    SENSITIVITY Recombinant
    MW (kDa) 115
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Erk5 (Thr218/Tyr220) Antibody detects recognizes active recombinant human Erk5. This antibody cross-reacts with phosphorylated Erk1 and Erk2. This antibody does not cross-react with phosphorylated p38 MAPK or SAPK/JNK.

    Species Reactivity:

    Human

    The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.

    Species predicted to react based on 100% sequence homology:

    Mouse

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr218/Tyr220 of human Erk5. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1-4). Like other MAPK family members, Erk5 contains a canonical activation loop TEY motif (Thr218/Tyr220) that is specifically phosphorylated by MAP2K5 (MEK5) in a growth-factor-dependent, Ras-independent mechanism (5-7). For example, EGF stimulation promotes Erk5 phosphorylation that induces its translocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (5,6). Erk5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoietic progenitor cells where it promotes survival and proliferation (7). In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival (8,9). Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function (10-12). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting Erk5 plays a critical role in vascular development and homeostasis (1,2).

    Alternate Names

    Big MAP kinase 1; BMK-1; BMK1; BMK1 kinase; ERK-5; ERK4; ERK5; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; MAPK7; Mitogen-activated protein kinase 7; MK07; PRKM7

    For Research Use Only. Not for Use in Diagnostic Procedures.
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