PTMScan® Pilot Phospho-Tyrosine Rabbit Monoclonal Antibody (P-Tyr-1000) Kit #14478
Additional Information
This product is intended for peptide enrichment and mass spectrometry analysis. To learn more about our Proteomics Kits and Services please answer a few questions for our Proteomics group.
Product Information
Product Usage Information
Cells are lysed in a urea-containing buffer, cellular proteins are digested by proteases, and the resulting peptides are purified by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using a PTMScan® Motif Antibody conjugated to protein A agarose beads. Unbound peptides are removed through washing, and the captured PTM-containing peptides are eluted with dilute acid. Reversed-phase purification is performed on microtips to desalt and separate peptides from antibody prior to concentrating the enriched peptides for LC-MS/MS analysis. CST recommends the use of PTMScan® IAP Buffer #9993 included in the kit.
PTMScan® Pilot Phospho-Tyrosine (P-Tyr-1000) Kit has a higher sensitivity and specificity magnetic bead version: PTMScan® HS Phospho-Tyrosine (P-Tyr-1000) Kit in 10-assay (#38572) or 3-assay (#29879) formats.
Storage
Protocol
Product Description
Background
Limited Uses
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