DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag) #14209
Product Information
Product Usage Information
This kit contains two bottles of 18 mL DNA Binding Buffer (#44364). Before first use, add 11.25 mL of isopropanol to each bottle. In addition, add 24 mL of ethanol to the DNA Wash Buffer (#10008) before first use.
Use the appropriately prepared buffers as directed in the respective SimpleChIP® Chromatin IP Kit, CUT&RUN Assay Kit, and CUT&Tag Assay Kit protocols.
Please note that if you are following the SimpleChIP® Chromatin IP or CUT&Tag protocol, you will have excess DNA Binding Buffer (#44364). This is intentional, as the kit includes sufficient DNA Binding Buffer to support the CUT&RUN protocol, which requires a larger volume of DNA Binding Buffer than the other protocols.
Storage
Product Description
This product is offered to enable DNA purification using spin columns with our CUT&RUN Assay Kits (#86652 and #84647).
This product is also offered to conveniently provide additional buffer reagents when using our SimpleChIP® (#9002, #9003) and SimpleChIP® Plus (#9004, #9005) Enzymatic Chromatin IP Kits, SimpleChIP® Sonication Chromatin IP Kit (#56383), and CUT&Tag Assay Kit (#77552). These kits provide all the reagents required for performing the respective assays, however there are instances where extra buffers are desired.
The DNA purification columns combine the convenience of spin-column technology with the selective binding properties of a uniquely designed silica membrane that allows for efficient recovery of DNA and removal of protein contaminants without the need for phenol/chloroform extractions and ethanol precipitations. After DNA purification, the enrichment of particular DNA sequences can be analyzed by a variety of methods.
Limited Uses
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