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In the CST video library, you can watch how-to videos from the Tech Tips series to improve your technique, research webinars presented by scientists from CST and other institutions, and videos relevant for specific applications or disease research areas.

The CST protocol for Fluorescent Multiplex Immunohistochemistry allows you to characterize 6 or more targets in one sample with flexible experimental design by employing a serial labeling strategy. In this video, Jen highlights five key protocol steps you can optimize to help get a balanced set of signals.
5 Tips to Start Your Multiplex IHC Optimization
How sample type, processing steps, and characteristics of the target protein can impact detection and inform protocol optimization in immunofluorescence.
A Guide to Detecting Your Favorite Protein by IF
This presentation recorded at SLAS describes the use of antibodies to probe targets in the TGF-β/Smad Signaling pathway using high throughput, automated quantitative ELISA and fluorescence-based microscopy assays.
A Multiscale Approach to Quantitatively Evaluate the SMAD Signaling Pathway
SignalStar® Multiplex IHC for spatial biology. Up to 8-plex protein detection generated in just 2 days. Watch the on-demand webinar.
Accelerate Spatial Discoveries Up to 70% Faster with SignalStar® Multiplex IHC
Watch this 3-D animation to see how flow cytometry enables the simultaneous characterization of intracellular signaling states and phenotyping of rare cell populations in a heterogeneous sample.
An Introduction to Flow Cytometry
This presentation showcases the use of antibodies immunofluorescence and flow cytometry, and the importance of validation.
Analysis of Cellular Signaling by Fluorescent Imaging and Flow Cytometry
Watch this webinar to learn about a streamlined workflow for generation of CD19 CAR-T cells using BioTechne analytical instrumentation and anti-CAR linker antibodies from CST.
Analytical Tools to Evaluate CAR-T Cell Signaling & Activation
Visualize immune cell identity, function, and spatial context with CST’s validated IHC antibodies. Maximize your research impact with precision tools.
Analyze Immune Cell Phenotype & Function
Is the performance an unconjugated antibody enough to validate a conjugated version of the same clone for your assay? Not necessarily! Learn why in this video.
Antibody Conjugates: What You Need to Know
Learn how phosphorylation, ubiquitination, & Atg8ylation impact autophagy, a key cellular process linked to health & disease. Watch the on-demand webinar featuring Reuben Shaw & Vojo Deretic.
Autophagy's hidden drivers: Exploring the role of post-translational modifications
This video highlights considerations for optimizing antibodies and adjusting the protocols when transfering protocols from manual to automated immunohistochemistry.
Autostainer IHC Protocol Transfer Q&A
Meet Gary Kasof PhD, who over his career has developed some of the most-cited antibodies antibodies for studying cell death. Gary also shares advice for young scientists.
Behind the Antibodies with Gary Kasof
Watch this webinar for applications, tips, and tricks for Simple Western, for fast, reliable, and hands-free automated western blotting using CST antibodies. See an overview of the CST-Bio-Techne partership, brining westerns into the 21st century.
Bringing the Western Blot into the 21st Century with Automated Simple Western – A Customer's Perspective
How does antibody validation affect your IHC results? Learn about the approaches scientists at CST use to validate antibodies for IHC in this video: cell pellets, multiple tissues, and treatments with activators, inhibitors, or blocking peptides.
Can You Trust Your Immunohistochemistry Results?
This video describes the application of robust multiplex imaging technologies to interrogate myeloid and lymphoid lineages to provide spatial insights of many cell types in the tumor microenvironment.
Capturing the Spatial Landscape of Tumor and Immune Cell Lineages in the Microenvironment of Human Cancer Tissues
Keep your cell and tissue culture free of contamination - watch this how-to video for tips and advice from our scientists.
Cell Culture: Aseptic/Sterile Technique
1.ChIP和ChIP seq的区别和应用范围； 2.ChIP和ChIP seq 实验的操作步骤和成功要素； 3.酶解和超声的优缺点及如何正确选择； 4.如何选择合适的用于ChIP和ChIP seq的抗体； 5.CST 标准 DNA文库建立方法及ChIP-Seq测序数据分析解读
ChIP & ChIP-Seq实验原理与成功要素及NGS测序数据分析解读
Need to decide between different lysis buffers for your Western Blot (WB)? Before you start running your proteins with RIPA buffer, cell lysis buffer, or SDS, Sarah has some questions you should consider.
Choosing a Lysis Buffer for Western Blot (WB)
Learn how to select antibodies with application-specific validation to ensure reproducibility and success in your IF, IHC, and multiplex imaging assays.
Choosing the Right Antibody for Imaging Success
Watch this video to learn how CST® CUT&Tag kits, reagents, and antibodies fuel your research with consistent, reliable, and reproducible results.
Chromatin Profiling Just Got Faster with CUT&Tag
This video explains the roles of the bait and prey proteins in co-IP, and applications of immunoprecipitation for studying protein-nucleic acid interactions.
Co-IP Bait and Prey, and other applications of Immunoprecipitation (IP)
Learn how spatial biology and multiplex IHC can be used to understand the immune system's role in disease and therapy. This webinar covers assay design, antibody selection, and methods for various plex sizes.
Context is Key: Leveraging Spatial Biology in Immunology and Immunotherapy
Video: the updated CUT&RUN kit provides improved results, confirmed compatibility with fixed frozen cells, tissues, and primary cells, uses even fewer cells.
CST CUT&RUN Kit: Validated for Flexibility
该视频重点介绍肿瘤微环境当中缺氧代谢与血管生成相关的调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标作为参考。
CST肿瘤微环境主题系列讲座---第一讲：缺氧代谢与血管生成
该视频将重点介绍与细胞骨架、细胞基质动态调控及EMT相关的主要调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标作为参考。
CST肿瘤微环境主题系列讲座---第三讲：细胞粘附、细胞骨架、细胞基质、EMT
该视频重点介绍肿瘤免疫微环境相关的主要参与细胞、调节分子和信号通路，并为各位听众提供在上述领域的主要评价指标和新的研究方法作为参考。
CST肿瘤微环境主题系列讲座---第二讲：肿瘤免疫微环境
A comprehensive, step-by-step video of the CUT&RUN Protocol, from sample preparation to CUT&RUN DNA purification and qPCR quantification. Expert tips for critical steps and experimental design are highlighted to help you successfully complete the assay.
CUT&RUN Protocol: A Visual Guide
Understanding the functional mechanisms and signaling pathways that regulate activation of specific T cell subpopulations is crucial to optimizing effectiveness of the next generation of CAR-T therapies. Presented by Wendell Lim and Stanley Riddell.
Deciphering CAR-T Cells: Exploring Functional Mechanisms to Drive Next-Generation Immunotherapy
Understanding the underlying biology of fibrosis is critical for the diagnosis, treatment, and management of cardiac fibrosis, nonalcoholic steatohepatitis (NASH), and other fibrotic diseases
Deciphering Fibrosis: Drivers of Fibrotic Disease in the Liver and Heart
Drs. Michael Heneka and Christian Haass present recent findings on how signaling involving TREM2 in microglia triggers the innate immune response in neurodegenerative diseases including Alzheimer's.
Deciphering Microglia: Exploring Cellular Pathways in Neurodegenerative Diseases
Innovative anti-linker monoclonal antibodies from CST eliminate the need to develop unique detection reagents for every new chimeric antigen receptor (CAR) molecule developed, speeding detection in flow cytometry panels.
Detecting CAR Expression with Versatile, Innovative Antibodies
A comprehensive, step-by-step video of the CUT&RUN DNA Library Prep Protocol for Illumina systems, picking up after the CUT&RUN protocol video. Key protocol steps and tips are highlighted.
DNA Library Prep for CUT&RUN: A Visual Guide
Designing or troubleshooting flow cytometry experiments to analyze intracellular signaling? Here’s what you need to know.
Experimental Design: Analyzing Intracellular Signaling With Flow Cytometry
Good controls can help you get your experiment back on track if something goes wrong, and can help you have confidence in your results. In this Tech Tip video, Ginny goes over three types of controls for your immunofluorescence (IF) experiment: positive controls, endogenous controls, and negative controls.
Experimental Design: Controls for Immunofluorescence (IF)
Explore the difference between two major types of fixatives for immunofluorescence: crosslinking aldehydes including formaldehyde, and alcohols including methanol. John describes how choice of fixative can impact the structure of epitopes and the fluorescent properties of the sample itself, and affect your results.
Formaldehyde vs. Alcohol Fixation for Immunofluorescence (IF)
To enable surface characterization of chimeric antigen receptors (CARs), scientists at CST developed versatile and specific antibodies that detect the linker sequence of scFv domains. Watch the poster presentation from AACR.
Generation and Validation of Anti-Linker Monoclonal Antibodies for the Surface Detection of scFv-based CARs
Learn how to boost sensitivity and reproducibility in protein detection to tackle low-abundance targets where traditional western blotting fails.
Going Beyond the Limits of Traditional Westerns
Learn how choice of antibody diluent can affect your IHC results in this Tech Tip with Chris. Differences in antibody diluent properties can influence antibody-epitope interactions and specificity, and ultimately, the strength of your staining.
How Does Antibody Diluent Affect Staining in Immunohistochemistry (IHC)?
More antibody isn't always better! The optimal amount of antibody to use in a ChIP assay varies, depending on the antibody's affinity, sensitivity and specificity. See how the amount of antibody used can affect your Chromatin Immunoprecipitation (ChIP) results in this Tech Tip featuring Fang.
How Much Antibody Should I use in ChIP assays?
Adding intracellular signaling targets to your flow cytometry analysis? You'll need to fix and permeabilize your cells to allow antibodies to access their epitopes. Rob explains the protocol variations.
How to Fix and Permeabilize Cells for Flow Cytometry
Reliably identify senescence signaling in your samples using biomarkers with a 3-stage approach outlined in this video. No single assay is sufficient, but screening, co-staining, and verification with multiple biomarkers can improve your confidence.
How to Identify Senescence in Cells and Tissue
When setting up Immunohistochemistry (IHC) experiments, cell pellet controls can be used to test antibody specificity and tell you whether your protocol is working or needs to be adjusted. In this Tech Tip, Chris highlights situations where cell pellet controls can be valuable.
How to Incorporate Cell Pellet Controls in IHC Experiments
The goal of this video is to guide you through chromatin fragmentation, a critical step in a Chromatin Immunoprecipitation (ChIP) experiment. It is important to consider whether the target protein is a histone modification, transcription factor, or transcription co-factor since each protein binds differently to DNA. Due to these binding differences, additional measures should be taken into account when optimizing chromatin fragmentation for each protein class, whether performing sonication or enzymatic digestion.
How to Optimize your Chromatin Fragmentation for ChIP
Antigen retrieval can influence the strength of staining obtained in IHC. In this Tech Tip, Franny and Abigail show you why antigen retrieval (AR) is important for immunohistochemistry (IHC), and give you tips on how to set up Heat Induced Epitope Retreival in your microwave.
How To Perform Antigen Retrieval (AR) in Immunohistochemistry (IHC)
Protein enrichment by immunoprecipitation is frequently carried out prior to analysis. In this Tech Tip, Sarah presents considerations for choosing antibodies and beads for IP.
How to Select an Antibody for Immunoprecipitation
帮助科研工作者更好地理解 IHC 在疾病诊断中的核心作用，通过应用实例讨论 IHC 技术操作过程、结果分析中的细节和注意事项。介绍 mIHC 的原理方法，并以研究实例讨论 mIHC 在疾病研究中的价值。
IHC 和 mIHC 在疾病研究诊断中的方法介绍及注意事项
This video provides a step-by-step guide to the immunohistochemistry protocol for paraffin-embedded tissue tissue sections, used by scientists in Cell Signaling Technology's IHC group.
Immunohistochemistry Protocol for Paraffin-Embedded Tissue Sections
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (Japanese Audio)
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (Japanese Audio)
Watch this video for a step-by-step demonstration of the Immunoprecipitation Protocol for Native Protein for Western Blot analysis. Includes guidance for experimental design, choosing antibodies and beads, and tips and tricks for successful protein IP.
Immunoprecipitation Protocol: A Visual Guide
Researchers have been investigating several potential avenues to counter tumor immunosuppression and re-activate the immune system. One of these is CAR-T therapy, which is designed to harness the potent cytotoxicity of T cells by re-engineering their surface receptors. This video gives an overview of this exciting field. What’s on the horizon for the CAR-T therapy field, and what challenges are researchers and clinicians going to tackle to enable the next success? We’ll explore this exciting frontier in part two of our CAR-T video series.
Immunotherapy and CAR-T Cells
In this video, our experts provide protocol guidance for lysate preparation steps prior to protein electrophoresis and western blotting.
Impacts of Lysis Buffer and Sonication on Western Blotting Outcomes
Discover advanced methods for studying TREM2 and downstream signaling in human-relevant iPSC-derived microglia to advance Alzheimer’s disease research.
Interrogating Cell Signaling Pathways in iPSC-Derived Microglia
Learn how InTraSeq® Single Cell Analysis enables unbiased quantitation of RNA alongside intracellular and cell surface proteins at the single-cell level.
InTraSeq® Single Cell Analysis: A Multimodal Single-Cell Method for Simultaneous Measurement of RNA and Proteins to Uncover New Single-Cell Biology
By combining single cell analysis of intracellular protein phosphorylation states with RNAseq, InTraSeq technology provides deeper understanding of cellular heterogeneity and function.
InTraSeq® Single Cell Analysis: Get More Data from Your scRNAseq
To characterize activation of immune cells, measurement of intracellular signaling using phospho-specific antibodies combined with immune phenotyping using surface markers is ideal. Owing to the unique capability of flow cytometry to measure events at a single-cell level, “Phospho Flow” allows for the identification of rare signaling events during processes such as co-stimulatory signaling and manipulation of checkpoint inhibition.
Mastering Phospho-Flow: Immune Cell Activation
How does choice of blocking protein - nonfat dry milk vs bovine serum albumen (BSA) - affect your Western Blot (WB) results? In this Tech Tip, Srikanth explains our protocol recommendations to reduce background caused by nonspecific binding.
Milk or BSA? Choosing a Blocking Protein for Western Blotting (WB)
How to combine modulators with selection of antibody targets and controls in western blot experiments.
Modulating Signaling Activities with Activators & Inhibitors for Western Blot Experiments
Advances in multiplex IHC using Opal and validated CST rabbit primary antibodies to reliably stain for 6 markers in a mouse model of breast cancer; using Akoya Bioscience's Phenoptics platform to perform quantitative analysis of tumor biology.
Multiplexing on Mouse tissue using CST antibodies and OPAL IHC method
Targeted protein degradation selectively alters protein abundance by co-opting the cellular degradation machinery. The degradation tag (dTAG) system can be used for target validation and mechanistic studies.
New Horizons for Protein Degradation: Immediate, Selective Protein Control Using the dTAG System
Unlock Th17 cellular mechanisms with InTraSeq single-cell analysis. Watch the webinar to learn about RNA & protein co-quantification & more.
No More (Data) Secrets: Unlock the Molecular Mechanisms of Pathogenic and Non-Pathogenic Th17 Cells
Rapidly advancing single-cell and multi-omic technologies generate complex datasets, but how can they drive deeper insights and actionable discoveries?
Not All Antibodies Are Created Equal: Why Application‑Specific Validation Defines Single‑Cell Insights
Watch this webinar to learn how the new CAR linker antibodies can interrogate the expression of an entire panel of CARs.
Novel Antibodies for the Characterization of CAR-T Cells
In this video, our flow expert compares different cell dissociation methods and how to assess their effects on cell viability, protein expression, and signaling.
Preparing for Flow: Cell Dissociation Methods
This video describes considerations for optimizing capture and detection antibody pairs to enable consistent ELISA results.
Reliable Results with Optimized ELISA
This video shows how you can amplify up to 8 targets in mouse or human tissue in just 2 days, with flexible panel design and high-quality antibodies from CST.
SignalStar® Multiplex IHC for Spatial Biology
Eliminate technical noise in your chromatin profiling. Watch our webinar to master sample normalization for quantitative CUT&RUN and CUT&Tag data.
Spike-in Controls: Generate Quantitative CUT&RUN and CUT&Tag Data You Can Trust
This video highlights three essential tips for splitting mammalian cell cultures, and explains how to use ratios or a cell counter to when propagating cells.
Splitting Cells: Why It's Important and 3 Essential Tips
By selectively targeting previously inaccessible oncogenic proteins for degradation, researchers are enabling functional studies with superior kinetic resolution and discovering new therapeutic targets.
Targeted protein degradation: Controlling and assessing therapeutic targets to advance oncology drug discovery
Know what to look for and dig deeper into the validation data for your antibody to avoid the frustration of ambiguous or misleading results.
The CST Hallmarks of Validation
Watch this webinar on the critical role of antibody validation in experimental success. Learn strategies to ensure immunoassay reproducibility and optimize your protocols.
The Importance of Antibody Validation and Its Impact on Your Research
Learn insider tips and tricks for successful SignalStar® Multiplex IHC experiments. This video covers critical steps, troubleshooting, and optimization for both manual and automated protocols to help you maximize experimental success and confidently interpret your results.
Tips & Tricks for Successful SignalStar® Experiments
What antibodies can be used in a CUT&RUN protocol? What controls should be included to measure signal-to-noise? These and other frequently asked questions are answered in this edition of CST Tech Tips.
Top 5 Frequently Asked CUT&RUN Questions
本次研讨会将通过介绍CST Western Blot标准操作步骤，向您阐释WB操作中的关键点及注意事项，特别是影响最终结果呈现的样本制备和抗体孵育与检测。通过真实失败案例，如无信号、高背景、条带大小不正确、条带弯曲拖尾等，分析WB常见的问题及原因，并提供相应的解决策略。
WB标准操作流程与常见问题解答
Lysate controls for Western Blot (WB) experiments are from a sample with known expression of your protein of interest, giving you an idea of how much signal to expect from your experimental samples. In this Tech Tip, Alex discusses how to use positive lysate controls, how they differ from loading controls, and considerations for phospho-specific antibodies.
Western Blot (WB) Experimental Design: Choosing Positive Controls
Common problems encountered in western blotting include low signal or high background. This video helps you troubleshoot problems in your Western Blot experiment, tips to help diagnose problems, and provides solutions to ensure you get the expected results in the shortest amount of time.
Western Blot Troubleshooting Guide
Western Blot Troubleshooting Guide (Japanese Audio)
Western Blot Troubleshooting Guide (Japanese Audio)
Western Blot Troubleshooting Guide (CN)
Western Blot Troubleshooting Guide 实验操作步骤视频解析
Western blotting (WB) is widely used to analyze specific protein expression in cell or tissue extracts. We understand that western blotting experiments are time consuming and critical to your research progress. For that reason, we provide optimized protocols along with reference information and technical support to make your western blotting experience successful.
Western Blotting Protocol
Western Blotting Protocol (CN)
Western Blotting Protocol 蛋白印迹实验步骤
Western Blot Basic Webinar (JP)
ウェスタンブロッティング (WB) の基礎
ChIP Basic Webinar (JP)
クロマチン免疫沈降 (ChIP) の基礎
Immunohistochemistry Basic Webinar (JP)
免疫組織化学染色 (IHC) の基礎
Immunofluorescence Basic Webinar (JP)
免疫蛍光染色 (IF) の基礎
本讲座将为大家系统地介绍cGAS-STING信号通路在天然免疫和肿瘤免疫研究领域的广泛作用，以及作为干预靶标的巨大潜力。
具有巨大潜力的天然免疫和肿瘤免疫干预靶标——cGAS-STING信号通路
本场讲座将逐一介绍多种细胞程序性死亡（凋亡、自噬、程序性坏死、细胞焦亡）相关信号通路关键蛋白和研究策略，并就多种细胞程序性死亡形式之间的联系和转化展开讨论，为大家全面认识细胞程序性死亡和开展系统研究提供参考。
凋亡相关信号通路研究进展和关键蛋白研究策略
本讲座全面讲解如何深入开展信号通路的研究，从大数据挖掘到锁定目标信号通路，进一步探讨疾病分子机制，并涉及到相关主流的实验技术，如修饰蛋白质组数据库使用、WB、ChIP、IHC、PTMScan蛋白质翻译后修饰高通量筛选技术等。
如何让你的发现可靠可重复？千里之行始于足下
作为指导方针，本次讲座将与您分享CST流式细胞术专家在日常的试验工作中经常使用的经验和技巧。
掌控Phospho-Flow： 巧用细胞内、外流式细胞术优化免疫细胞活化分析
随着流式细胞术近几年的飞速发展，它已成为生物科学领域最有力的分析技术之一。对于这一技术，有的人却望尘莫及，问题层出不穷，比如，胞内染色染不出，找不到合适的抗体，修饰蛋白不知道怎么刺激等等。其实，跳出墨守陈规的流式应用，深度挖掘更深层次的流式实验，助力科研及临床的发展，是所有 Flower 为之奋斗的目标。本讲座将带您了解 Cell Signaling Technology (CST) 流式产品在细胞通路研究中的独到之处，让我们一起在广阔的流式世界中，携手同行，共辟蹊径。
流式（Flow）入通路，蹊径何处寻 | Webinar
Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections (CN)
用于石蜡包埋的组织切片的免疫组织化学实验步骤 | Immunohistochemistry Protocol for Paraffin-embedded Tissue Sections
细胞焦亡与其他多种细胞程序性死亡形式之间存在相互联系和转化，共同构成细胞程序性死亡的复杂体系，维持细胞和机体的内稳态。
细胞焦亡信号通路的关键蛋白和研究动态
细胞程序性死亡(Programmed Cell Death, PCD)，是指细胞接受某种信号或受到某些因素刺激后，为了维持内环境稳定而发生的一种主动性消亡过程。它
细胞程序性死亡相关信号通路关键蛋白和研究策略
该视频将将逐一介绍参与自噬信号通路的关键分子，包括ULK1 、Beclin1、LC3、Atg5，ATG12，P62等
自噬相关信号通路关键蛋白和研究策略
本场 Webinar 详细介绍 PTMScan® 技术，并结合精准医学中的应用案例介绍如何选择和使用 PTMScan® 相关产品。
蛋白翻译后修饰组学技术（PTMScan®）在精准医学中的应用