Technical Support
Results (680)
What is your recommended procedure for antigen retrieval with a pressure cooker?
For antigen retrieval in a pressure cooker, bring slides submerged in buffer up to 125C, maintain for 30 seconds, then cool to 90C and maintain that for 10 sec. Remove the container from the device an...
How should I prepare my samples to detect phospho-Smad1/5/9?
- Our Phospho-Smad1/5 (Ser463/465) (41D10) Rabbit mAb #9516 and Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) Rabbit mAb #13820 are performance tested on extracts pr...
Are the Protein G Agarose Beads #37478 compatible with goat antibodies?
While we have not tested our Protein G Agarose Beads #37478 with goat antibodies in-house, goat IgG has a strong binding affinity to protein G. Therefore, our Protein G Agarose Beads should be compati...
What is the difference between Normal Rabbit IgG #2729 and Rabbit (DA1E) mAb IgG XP® Isotype Control #3900?
Our Normal Rabbit IgG #2729 is an unconjugated rabbit polyclonal antibody that is routinely used as a non-specific rabbit IgG control for IP and ChIP, whereas our Rabbit (DA1E) mAb IgG XP® Isotype Con...
How can I fragment the input DNA for NG-seq if I do not have access to a sonicator?
If a sonicator is not available and downstream NG-seq analysis is desired, an enzymatic input DNA fragmentation protocol can be found on the The specificity of our NT5E/CD73 (D7F9A) Rabbit mAb #13160 has been confirmed by siRNA/shRNA-depletion in the following papers: Yes, please select “Human” in the first step of the SignalStar Multiplex IHC Panel Builder ... Each buffer in the Cell Fractionation Kit #9038 contains detergents to help isolate different cellular fractions. The Cytoplasmic Isolation Buffer (CIB) contains a non-ionic detergent used as a cell p... Yes, you can use primers from the Illumina Nextera Library preparation system or self-designed primers to amplify the DNA library generated from the CUT&Tag DNA using the CUT&Tag Assay Kit #77... CST antibodies undergo thorough validation processes to ensure reliable performance for every approved application. This includes testing different protocol variations to identify the most effective o... The Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 is a mouse mAb from a single clone, whereas the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 is a mix of rabbit mAbs from multiple clon... Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are stability tested by ex... For custom (carrier-free) orders, many antibodies are available pre-formulated in PBS without BSA or glycerol. This carrier-free formulation is amenable to conjugation and can be conjugated to fluorop... Yes, please select “Mouse” in the first step of the SignalStar Multiplex IHC Panel Builder ... We have several antibodies for detecting β-actin that are validated for IF-IC with human samples. These include: When selecting an antibody for use in CUT&RUN, we recommend starting with a ChIP or ChIP-seq-validated antibody; however, we have found that not all ChIP-validated antibodies work in the CUT&am... The Concanavalin A beads can clump together rather easily, especially when the cells become lysed. To avoid this, make sure your cells are healthy and be sure to treat them very gently during the cell... The selection of a phospho-AMPKα antibody depends on the subunit of interest. Cell Signaling Technology has four antibodies that target phospho-AMPKα:Can you provide data that confirms the specificity of your NT5E/CD73 (D7F9A) Rabbit mAb #13160?
PMID: 33086655 Figure 3 (Do you have human-reactive antibodies available for SignalStar™ Multiplex IHC?
Why do I need to use the three buffers in the Cell Fractionation Kit #9038?
Can I use primers from the Illumina Nextera Library preparation system or design my own primers to amplify the DNA library generated from the CUT&Tag Assay Kit #77552??
Can I modify the CST-recommended protocol for a given application?
How do the Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411 and the Phospho-Tyrosine (P-Tyr-1000) MultiMab™ Rabbit mAb mix #8954 compare in terms of their sensitivity and specificity?
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Can I order a custom formulation of an antibody?
Do you have mouse-reactive antibodies available for SignalStar™ Multiplex IHC?
Which β-actin antibody do you suggest for immunofluorescence-immunocytochemistry (IF-IC) in human samples?
What is the best way to choose an antibody for my CUT&RUN assay if I cannot find a CUT&RUN validated antibody for my target protein?
Should I be concerned that my Concanavalin A beads "clump together" in some of my CUT&RUN experiments?
Which phospho-AMPKα antibody do you suggest for my western blot experiment?
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with mouse samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with mouse samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
When comparing my SignalStar™ staining to the chromogenic staining on a serial section, I see more positive cells. How do I know if this excess staining is correct?
During the course of optimization, we’ve found that fluorescent staining may show higher %-positivity than chromogenic staining. To ensure any excess staining is specific, confirm that the correct sub...
Which cleaved caspase-3 (Asp175) antibody do you suggest for paraffin-embedded immunohistochemistry (IHC-P) with human samples?
We have several antibodies for detecting caspase-3 when cleaved at Asp175 that are validated for IHC-P with human samples. These are:
- Cleaved Caspase-3 (Asp175) Antibody #9661
- Cleaved ...
I don’t see my target of interest in your menu of available antibodies for SignalStar™ Multiplex IHC. Can I still use it in my panel in some way?
SignalStar Multiplex IHC kits and reagents haven’t yet been validated for use with antibodies outside of our menu. We’re in the process of developing cust...