Technical Support Articles
Results (707)
Are LICOR buffers compatible with CST products in fluorescent western blot?
While we strongly urge customers to continue using our recommended blotting buffers, it has been found that the TBS based LICOR buffers also appear to be compatible with our produc...
My items say to store at -20°c but they were shipped in an envelope. Is it ok to use?
Yes, it is safe to use these items. Since 2003, CST has been shipping antibodies at room temperature when possible to reduce waste from packing materials. All CST antibodies are st...
What if my yield of CUT&Tag DNA is too low to see on an Agilent Bioanalyzer or TapeStation system?
Due to the expected and typically low yield of CUT&Tag DNA, we recommend using all 30 µL of the CUT&Tag DNA sample (from Unfortunately, we are unable to provide the Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product. This product is m... In-house, we tested AMPK Control Cell Extracts #9158 and mouse liver extract, blotting with AMPKα2 Antibody #2757 (approved for human and monkey) and a competitor AMPKα2 antibody (... Our LC3 antibodies provide a good readout for autophagy, as indicated by changes in the abundance of LC3-II. However, our LC3 antibodies may have stronger reactivity with the type ... The 10X Fixative Solution found in our Senescence β-Galactosidase Staining Kit #9860 contains 20% formaldehyde and 2% glutaraldehyde in 10X PBS. While we recommend 1mg of tissue per assay in most cases, we have had some success with as little as 0.5 mg of tissue per assay. Easier tissue types, such as liver, or easier targe... The Cellular Glutathione Detection Assay Kit #13859 detects only the reduced form of glutathione. It is unable to detect the oxidized form. Our PTMScan Pan-Methyl Lysine Kit #14809 will enrich for all three forms of methylated lysine: mono, di and tri-methyl lysine. To reduce the complexity of the search space, it is n... No, we do not observe any sequence bias in our CUT&RUN assays. Free MNase may have some sequence bias in the genome, however, in CUT&RUN, the MNase is fused with pAG and re... When developing the Malachite Green Phosphate Detection Kit #12776, we successfully used the Millipore Centrifuge filters AMICON ULTRA 0.5mL, 10,000 NMWL, product # UFC501096. A BCA protein assay will likely not provide an accurate concentration for our antibodies formulated in glycerol, due to the presence of BSA and incompatibility with a higher percen... TET2 is post-translationally modified (https://www.phosphosite.org/proteinAction.action?id=22594&showAllSites=true). Although the theoretical molecular weight is 223.8 kDa thes... Our Akt (pan) (C67E7) Rabbit mAb #4691 does not detect over-expressed or endogenous levels of PKC. Our Cleaved PARP (Asp214) (D64E10) XP Rabbit mAb #5625 is designed to be specific for the large fragment (89 kDa) of human PARP1 protein that is produced by caspase cleavage at Asp... Based on homology, our β-Tubulin Antibody #2146, β-Tubulin (9F3) Rabbit mAb #2128, β-Tubulin (D3U1W) Mouse mAb #86298, and β-Tubulin (D2N5G) Rabbit mAb #15115 should detect β-Tubul... We observe multiple bands at or around 33-35 kDa in cells with high STING protein expression, such as THP-1 and HDLM-2. Regrettably, we are not certain about what the lower molecul... There is 50 µl of each antibody component that is listed in the StemLight Pluripotency Antibody Kit #9656. The kit components are pre-optimized for parallel use in immunofluorescen... Please see Maniaci, C. et al. (2017) Nat. Commun. 8, 830 (PMID: 29018234; https://pubmed.ncbi.nlm.nih.gov/29018234/) and Frost, J. et al. (2021) J. Biol. Chem. 297, 100910 (PMID: 3... For western blotting, we recommend mixing the primary antibody in the appropriate dilution buffer (see product datasheet) with at least 1.0 ug of peptide per 1.0 ul of primary anti... The samples shown in the web image for our Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833 were prepared as follows: We offer 3 MitoTracker® products, MitoTracker® Green FM #9074, MitoTracker® Deep Red FM #8778 and MitoTracker® Red CMXRos #9082. MitoTracker® Deep Red FM #8778 and MitoTracker® R... The background or number of non-PTM peptides in each enrichment is primarily due to non-specific interactions with the beads the antibody is conjugated to. Therefore, this backgrou... We do not recommend performing size selection of the DNA during library preparation for CUT&RUN or ChIP-seq. Based on our experience and feedback from customers, size selection... The antigens for our COX IV Antibody #4844 and COX IV (3E11) Rabbit mAb #4850 are 100% conserved with COX IV isoform 1. There is less than 20% homology of these antigens with COX I... The typical DNA yields from ChIP experiments range from 0.2-2 ng/µl when targeting transcription factors or cofactors and from 2-20 ng/µl when targeting histone modifications. If t... We apologize for any confusion regarding our EGF Receptor (D38B1) XP® Rabbit mAb #4267 and its recommendation for IHC on human tissue only. The species reactivity at the top of the... The antigen used to produce our Vav1 (D45G3) Rabbit mAb #4657 is 100% conserved in Vav1 isoforms 1, 2 and 3. Therefore, we would expect this antibody to recognize these three isofo... Our optimized digestion conditions for the CUT&RUN assay work well across multiple cell lines, both adherent and suspension. In addition, we have not observed significant chang...
Can I purchase your Sample Normalization Spike-In DNA (2 pg/µl) #29987 as a stand-alone product?
How much AMPKα2 protein is expressed in AMPK Control Cell Extracts #9158?
Why can't I detect LC3-I?
Are you able to disclose the ingredients of the fixative solution in the Senescence β-Galactosidase Staining Kit #9860?
What is the minimal amount of tissue required for a CUT&RUN experiment?
Does the Cellular Glutathione Detection Assay Kit #13859 detect both reduced and oxidized glutathione?
How should I search for the Pan-Methyl Lysine PTM in LC-MS/MS data when using your PTMScan® Pan-Methyl Lysine Kit #14809?
I have seen reports that MNase has some sequence bias. Do you see this in your CUT&RUN assays and does it affect the target protein-interaction DNA profiles you get?
Which desalting columns do you recommend to use with the Malachite Green Phosphate Detection Kit #12776 in order to remove contaminating phosphate from the cells?
Can I use a BCA assay to check the concentration of my antibody?
Why does the observed size versus the theoretical size of TET2 differ by western blot?
Does your Akt (pan) (C67E7) Rabbit mAb #4691 detect PKC?
Will your Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb #5625 also recognize the smaller PARP-1 fragments (40-55 kDa) that are generated during necrosis?
What β-Tubulin isoforms and subtypes are detected by CST β-Tubulin antibodies?
What is the identity of the extra band detected in THP-1 extract by your STING (D2P2F) Rabbit mAb #13647?
How much of each antibody is supplied in the StemLight™ Pluripotency Antibody Kit #9656 and how does this relate to the number of tests?
What are the three bands detected by western blot with your VHL Antibody #68547?
How should blocking peptides be used for western blotting?
How do I prepare the samples shown in the web image for your Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833?
1. Treat cells with 200 nM ...
Which MitoTracker® reagents are well-retained after fixation and which are only useful for live cell imaging?
Why are there background peptides in my PTMScan® experiment?
Do you recommend performing size selection of the DNA during library preparation for CUT&RUN or ChIP-seq?
Do your COX IV antibodies detect both isoforms of COX IV?
How much DNA is required to make my library for ChIP-seq?
Does your EGF Receptor (D38B1) XP® Rabbit mAb #4267 work for IHC on mouse samples?
What isoforms does the Vav1 (D45G3) Rabbit mAb #4657 detect?
Do I need to optimize the MNase digestion conditions in my CUT&RUN experiment for different cell types?