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  4. Organelle Markers

Organelle Markers

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Colocalization with one of the organelle-specific reagents listed here can confirm the subcellular location of a protein of interest. While these organelle markers serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Expression of these proteins may vary in different cells and tissues.

Cytoskeleton

No.NameApplicationsReactivity
8844β-Actin (13E5) Rabbit mAb (Alexa Fluor® 488 Conjugate)IF-IC, FH, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
9470β-Actin (13E5) Rabbit mAb (Alexa Fluor® 594 Conjugate)IF-ICH, M, R, Mk, B, Pg, (C, Dg, Hr)
8456Pan-Actin (D18C11) Rabbit mAbW, IHC-P, IF-ICH, M, R, Mk
8584β-Actin (13E5) Rabbit mAb (Alexa Fluor® 647 Conjugate)IF-IC, FH, M, R, Mk, B, Pg, (C, Dg, Hr)
8457β-Actin (D6A8) Rabbit mAbW, IF-IC, FH, M, R, Mk
4970β-Actin (13E5) Rabbit mAbW, IHC-P, IHC-F, IF-IC, FH, M, R, Mk, B, Pg, (Hm, C, Dg, Hr)
3700β-Actin (8H10D10) Mouse mAbW, IHC-P, IF-IC, FH, M, R, Hm, Mk, Dg
5332Desmin (D93F5) XP® Rabbit mAbW, IF-F, IF-ICH, M, R, (Mk)
13063Keratin 20 (D9Z1Z) XP® Rabbit mAbW, IHC-P, IF-IC, FH
4528Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate)IF-P, IF-IC, FH, R, Mk
4523Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate)IF-P, IF-IC, FH, R, Mk
4545Pan-Keratin (C11) Mouse mAbW, IHC-P, IF-P, IF-IC, FH, R, Mk
4898Keratin 7 (R458) AntibodyW, IF-ICH
4543Keratin 17 (D73C7) XP® Rabbit mAbW, IF-IC, FH, M, R, (Mk, Dg)
12434Keratin 17/19 (D4G2) XP® Rabbit mAbW, IHC-P, IF-ICH, M, R
8953Alexa Fluor® 555 PhalloidinIF-F, IF-ICAll
12956DyLight™ 650 PhalloidinIF-F, IF-ICAll
12877DyLight™ 594 PhalloidinIF-F, IF-ICAll
13054DyLight™ 554 PhalloidinIF-F, IF-ICAll
12935DyLight™ 488 PhalloidinIF-F, IF-ICAll
8940Alexa Fluor® 647 PhalloidinIF-F, IF-ICAll
8878Alexa Fluor® 488 PhalloidinIF-F, IF-ICAll
3623β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 488 Conjugate)IF-IC, FH, M, R, Mk, Dm, Z, B, (C)
3624β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 647 Conjugate)IF-IC, FH, M, R, Mk, Dm, Z, B, (C)
2116β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate)IF-ICH, M, Mk, Dm, Z, B, (C)
2128β-Tubulin (9F3) Rabbit mAbW, IHC-P, IF-IC, FH, M, R, Mk, Z, B, (C)
2146β-Tubulin AntibodyW, IHC-P, IF-IC, FH, M, R, Mk, Z, B, (X)
2148α/β-Tubulin AntibodyW, IHC-P, IF-IC, FH, M, R, Mk, Z, B
2125α-Tubulin (11H10) Rabbit mAbW, IHC-P, IF-IC, FH, M, R, Mk, Dm, B, (Dg, Pg)
3873α-Tubulin (DM1A) Mouse mAbW, IHC-P, IF-ICH, M, R, Mk
2144α-Tubulin AntibodyW, IHC-P, IF-IC, FH, M, R, Mk, Dm, B, (X)
5741Vimentin (D21H3) XP® Rabbit mAbW, IHC-P, IF-IC, FH, M, R, Mk

Cytoplasm

No. Name Applications Reactivity
8727 MEK1/2 (D1A5) Rabbit mAb W, IF-IC, F H, M, R, Mk, (Hm), Dm, (X, Z, B, Dg, Pg, Ce)
4694 MEK1/2 (L38C12) Mouse mAb W, IHC-P, IF-IC, F H, M, R, Mk

Centrioles / Centrisome

No. Name Applications Reactivity
5259 PCM-1 (Q15) Antibody W, IP, IF-IC, F H, (Mk)
5213 PCM-1 (G2000) Antibody W, IP, IF-IC, F H, M, (Mk)

Endoplasmic Reticulum

No. Name Applications Reactivity
8787 ER-Tracker™ Green (BODIPY® FL Glibenclamide) IF-IC  
5033 ERp72 (D70D12) XP® Rabbit mAb W, IF-IC, F H, M, R, Mk
3501 PDI (C81H6) Rabbit mAb W, IHC-P, IF-IC H, M, R, Mk

Golgi

No. Name Applications Reactivity
12480 GM130 (D6B1) XP® Rabbit mAb W, IP, IF-IC H, Mk
12290 RCAS1 (D2B6N) XP® Rabbit mAb W, IP, IF-IC, F H, M, R, (Mk)
13192 Golgin-97 (D8P2K) Rabbit mAb W, IP, IF-IC H, M

Mitochondria

No. Name Applications Reactivity
5318 AIF (D39D2) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (B, Dg)
7561 COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) IF-IC, F H, R, Mk, Z, B, Pg
8693 COX IV (3E11) Rabbit mAb (Alexa Fluor® 555 Conjugate) IF-IC H, R, Mk, Z, B, Pg
4853 COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) IF-IC, F H, R, Mk, Z, B, Pg
8692 COX IV (3E11) Rabbit mAb (Alexa Fluor® 594 Conjugate) IF-IC H, R, Mk, Z, B, Pg
4850 COX IV (3E11) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC, F H, R, Mk, Z, B, Pg
4844 COX IV Antibody W, IP, IHC-P, F H, M, R, Mk, B
12963 Cytochrome c (6H2.B4) Mouse mAb IP, IF-IC H, M, R
2024 Hexokinase I (C35C4) Rabbit mAb W, IP, IHC-P, IF-IC H, M

Endosomes

No. Name Applications Reactivity
3267 Caveolin-1 (D46G3) XP® Rabbit mAb W, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, B, Dg
3238 Caveolin-1 Antibody W, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Hm, Z, B, Pg
8522 Caveolin-2 (D4A6) XP® Rabbit mAb W, IP, IF-IC H, Mk
4796 Clathrin Heavy Chain (D3C6) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Pg)
2410 Clathrin Heavy Chain (P1663) Antibody W, IF-IC H, M, R, Mk, B, (Pg)
3288 EEA1 (C45B10) Rabbit mAb W, IP, IF-IC H, M, R
2411 EEA1 Antibody W, IP, IF-IC H, M, R, Mk
5589 Rab11 (D4F5) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Hm, Pg)
3547 Rab5 (C8B1) Rabbit mAb W, IF-IC H, M, R, Mk
2143 Rab5 Antibody W, IF-IC H, M, R, Hm, Mk
9367 Rab7 (D95F2) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk
5118 Rab9 (D52G8) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk

Autophagosomes

No.NameApplicationsReactivity
2011Atg12 Antibody (Mouse Specific)W, IP, IF-ICM
2010Atg12 Antibody (Human Specific)W, IP, IF-ICH
4599LC3A (D50G8) XP® Rabbit mAbW, IHC-P, IF-IC, FH, M, R, (Mk, Dg)
13118LC3A/B (D3U4C) XP® Rabbit mAb (Biotinylated)WH, M, R, (Mk, X, B, Dg, Pg)
13082LC3A/B (D3U4C) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)IF-IC, FH, M, R, (Mk, X, B, Dg, Pg)
12741LC3A/B (D3U4C) XP® Rabbit mAbW, IHC-P, IF-IC, FH, M, R, (Mk, X, B, Dg, Pg)
13611LC3A/B (D3U4C) XP® Rabbit mAb (PE Conjugate)FH, M, R, (X, B, Dg, Pg)
3868LC3B (D11) XP® Rabbit mAbW, IP, IHC-P, IF-IC, FH, M, R, (Mk, B, Pg)
2775LC3B AntibodyW, IF-IC, FH, M, R, (Mk, B, Pg)
7695SQSTM1/p62 (D10E10) Rabbit mAb (IF Preferred)IP, IF-ICH

Lysosome

No. Name Applications Reactivity
9091 LAMP1 (D2D11) XP® Rabbit mAb W, IP, IF-IC H, Mk

Nucleus

No. Name Applications Reactivity
5019 ASH2L (D93F6) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk, (Dm)
2196 ESET (C1C12) Rabbit mAb W, IP, IF-IC H, Mk
2718 Histone H2A.Z Antibody W, IP, IF-IC H, M, R, Mk, Z, (C, X, B)
2184 LSD1 (C69G12) Rabbit mAb W, IP, IHC-P, IHC-F, IF-IC H, M, R, Mk
2139 LSD1 Antibody W, IP, IHC-P, IF-IC, F H, M, R, Mk

Nuclear Envelope

No. Name Applications Reactivity
4777 Lamin A/C (4C11) Mouse mAb W, IP, IHC-P, IF-F, IF-IC, F H, M, R, Mk
12255 Lamin B2 (D8P3U) Rabbit mAb W, IP, IF-IC H, Mk
2598 NUP98 (C39A3) Rabbit mAb W, IP, IF-IC H, M, R, Mk

Nucleolus

No. Name Applications Reactivity
3833 eIF6 (D16E9) XP® Rabbit mAb W, IP, IF-IC H, M, R, Mk
2639 Fibrillarin (C13C3) Rabbit mAb W, IF-IC H, M, R, Mk

Peroxisomes

No. Name Applications Reactivity
12980 Catalase (D4P7B) XP® Rabbit mAb W, IP, IF-IC H

Intercellular Junctions

No.NameApplicationsReactivity
3199E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate)IF-P, IF-IC, FH, M, (B, Dg, Pg)
3195E-Cadherin (24E10) Rabbit mAbW, IHC-P, IHC-F, IF-IC, FH, M, (B, Dg, Pg)
7687E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate)IF-ICH, M, (Dg, Pg)
13116N-Cadherin (D4R1H) XP® Rabbit mAbW, IP, IHC-P, IF-ICH
2189P-Cadherin (C13F9) Rabbit mAbW, IF-ICH, (Mk)
2500VE-Cadherin (D87F2) XP® Rabbit mAbW, IP, IF-IC, FH, Dm, B, Pg, (Mk)
3512Connexin 43 AntibodyW, IHC-P, IHC-F, IF-F, IF-ICH, M, R, Mk, Z, (Dg, Pg)
2847ZO-2 AntibodyW, IF-ICH, M, R, Mk, B, Dg
3704ZO-3 (D57G7) XP® Rabbit mAbW, IF-ICH

Applications Key: W=Western blot  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)  IF-F=Immunofluorescence (Frozen)  IF-P=Immunofluorescence (Paraffin)  F=Flow cytometry 
Reactivity Key: H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  Dm=D.melanogaster  X=Xenopus  Z=Zebrafish  B=Bovine  Dg=Dog  Pg=Pig  Hr=Horse  Ce=C.elegans  All=All Species Expected 
Species enclosed in parentheses are predicted to react based on 100% sequence homology.