PerCP-Cy®5.5 Antibody Conjugates
Leverage the tandem fluorophore PerCP-Cy®5.5 for improved photostability and brightness over PerCP alone. Due to the resonance energy transfer from PerCP to Cy5.5, you can enhance the utility of your 488 laser line, making bright PerCP-Cy®5.5 conjugated antibodies an excellent choice for multiplexed flow cytometry workflows and assays targeting less abundant antigens.
Attributes
Excitation: 482 | Emission: 690 |
Laser Line: 488 nm | Common Filter Set: 695/40 |
Brightness: 4 | Relative Staining Index: Medium |
PerCP-Cy®5.5 conjugated antibodies have a far-red emission spectrum (solid red) with reduced emission overlap with many commonly used fluorophores. With an excitation maximum of 482 nm (dashed red line), the same laser line can be used with other fluorophores, such as FITC or PE, for easy integration into multicolor panels.
Applications in Research
Several properties of PerCP-Cy®5.5 make it a powerful research tool, such as:
- Brighter than PerCP alone due to improved energy transfer. This makes it ideal for detecting low-abundance markers or applications requiring a strong signal strength with limited autofluorescence.
- Excitable by the standard 488 nm laser, it is a convenient choice, as no additional lasers or modifications to equipment are required.
- Causes less Fc receptor-mediated nonspecific staining than other standard tandem fluorophore conjugates.
Resistance to photobleaching makes PerCP-Cy®5.5 more suitable for applications where samples are exposed to light for extended periods, including:
Achieve clarity when used with green and blue dyes
Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (PerCP-Cy®5.5 Conjugate) #90088 (right) compared to concentration-matched Rat Isotype Control (PerCP-Cy®5.5 Conjugate) #79201 (left), co-stained with CD44 (IM7) Rat mAb (violetFluor™ 450 Conjugate) #53289.
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use PerCP-Cy®5.5 antibody conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our PerCP-Cy®5.5 Conjugated Antibodies
Survey our catalog of validated PerCP-Cy®5.5 antibody conjugates, or take advantage of our custom conjugation services and let us build what you need for your research.
Related or Alternative Fluorophores
Brilliant Blue 700 | NovaFluor Blue 690 | mFluor Blue 660 |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. Cy is a registered trademark of GE Healthcare. mFluor is a trademark of AAT Bioquest, Inc. mFluor is manufactured by AAT Bioquest, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.