PE-Cy®7 Antibody Conjugates

Enhance your flow cytometry experiments with PE-Cy^®7 conjugates, offering exceptional brightness and far-red emission for minimal background interference. Compatible with widely available laser lines, PE-Cy^®7 provides a strong signal that excels in multicolor panels by reducing spectral overlap with fluorophores in the visible spectrum.

Flow cytometric analysis of live human peripheral blood mononuclear cells using CD16 (3G8) Mouse mAb (PE-Cy^®7 Conjugate) #23290 and co-stained with NCAM1 (CD56) (MY31) Mouse mAb (APC Conjugate) #51997 (right), compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PE-Cy^®7 Conjugate) #79339 (left).

Attributes

The excitation spectrum (dashed red line) and emission spectrum (solid red) for PE-Cy^®7 are shown above. Take advantage of the large Stokes shift and far-red/NIR emission, enabling multifluor excitation with the common 488, 532, 561 nm laser lines.

Applications in Research

PE-Cy^®7 is an ideal fluorophore for flow cytometry and immunophenotyping applications. Its far-red emission and exceptional brightness allow for high-sensitivity target detection with minimal spectral overlap.

• Target low-abundance markers or rare cell populations by leveraging PE’s brightness with Cy7’s far-red emission.
• PE-Cy^®7 harnesses energy transfer between PE and Cy^®7, maximizing laser compatibility and allowing the analysis of additional markers in high-dimensional experiments.
• Equally bright as PE conjugates, PE-Cyanine7 can be used alongside green, orange, and far-red fluorochromes, minimizing compensation adjustments in multicolor panels.
• Due to its susceptibility to photo-induced degradation, care must be taken to avoid photobleaching.

PE-Cy®7 is broadly compatible with varied detection systems, laser lines, and filter sets, making it suitable for multiplexed applications such as:

• Flow cytometry and spectral flow cytometry
• Immunophenotyping
• Western blotting

Optimize your flow cytometry readouts

Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (PE-Cy^®7 Conjugate) #61885 (right) compared to concentration-matched Rat Isotype Control (PE-Cy^®7 Conjugate) #43426 (left), co-stained with CD44 (IM7) Rat mAb (violetFluor 450 Conjugate) #53289.

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use PE-Cy^®7 conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our PE-Cy^®7 Conjugated Antibodies

Need ready-made PE-Cy^®7 primary antibody conjugates? Find what you need from our extensive catalog of validated products, or take advantage of our custom conjugation services and let us build the perfect tool for you.

Related or Alternative Conjugates

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology and CST are registered trademarks of Cell Signaling Technology, Inc. Cy is a registered trademark of GE Healthcare. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}