PE-Cy®5 Antibody Conjugates

Take advantage of the superior brightness and far-red emission of PE-Cy^®5 conjugates to optimize your experimental workflows. As one of the brightest fluorophores available, it is particularly useful for labeling low-abundance targets in multiplexed flow cytometry assays. While sensitive to photobleaching, PE-Cy^®5 excels in experimental setups requiring a strong signal where exposure to light is brief.

Flow cytometric analysis of live human peripheral blood mononuclear cells using CD44 (IM7) Rat mAb (PE-Cy^®5 Conjugate) #94170 (solid line) compared to concentration-matched Rat (LTF-2) mAb IgG2b Isotype Control (PE-Cy^®5 Conjugate) #66005 (dashed line).

Attributes

The excitation spectrum (dashed red line) and emission spectrum (solid red) for PE-Cy^®5 are shown above. As a tandem fluorophore, PE-Cy^®5 boasts a large Stokes shift and exceptional brightness, allowing you to achieve excitation with standard 488, 532, and 561 nm laser lines.

Applications in Research

PE-Cy^®5 can be a powerful tool for your research when deployed in various experimental setups and is an optimum choice when:

• Conjugated to antibodies to identify specific immune cells (i.e. CD3, CD4, CD8, and CD19), making it ideal for detecting rare or low-abundance markers within a population.
• Used in multicolor experiments when precise detection and minimal background interference are required.
• Versatility is needed to provide a bright signal in applications using standard laser lines and commonly used fluors like FITC, PE, and APC.

PE-Cy^®5 is best deployed in multiplexed flow cytometry workflows but can be suitable for a range of applications:

• Flow cytometry and spectral flow cytometry
• Immunofluorescence
• Spatial biology
• Immunophenotyping

Perform live cell sorting with precision

Flow cytometric analysis of live mouse splenocytes using CD45R/B220 (RA3-6B2) Rat mAb (PE-Cy^®5 Conjugate) #16935 (solid line) compared to concentration-matched Rat Isotype Control (PE-Cy^®5 Conjugate) #66005 (dashed line).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use PE-Cy^®5 conjugates. CST uses a stringent and reproducible manufacturing process that results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our PE-Cy^®5 Conjugated Antibodies

Are you looking for ready-made PE-Cy^®5 conjugates? Browse our catalog of validated products, or leverage our custom conjugation services to build the tool you need to accelerate your research.

Related or Alternative Conjugates

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology and CST are trademarks or registered trademarks of Cell Signaling Technology, Inc. Cy is a registered trademark of GE Healthcare. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}