Phycoerythrin (PE) Conjugated Antibodies

Get unmatched sensitivity with PE conjugated antibodies, one of the brightest fluorophores available. With a strong yellow-orange emission and excitation by standard laser lines, PE delivers a robust signal ideal for detecting low-abundance markers and rare populations. Its superior brightness and versatility make PE essential for flow cytometry, immunophenotyping, FACS, and more.

Flow cytometric analysis of Jurkat cells using p27 Kip1 (D69C12) XP^® Rabbit mAb (PE Conjugate) #12184 and DRAQ5 #4084 (fluorescent DNA dye).

Attributes

The excitation spectrum (dashed yellow line) and emission spectrum (solid yellow line) for PE are shown above. PE is one of the most sensitive fluorophores available, with a near-perfect quantum efficiency. Excitable by standard 488, 532, and 561 nm lasers, PE conjugated antibodies are ideal for flow cytometry and multicolor panels, enabling precise detection of your target.

Applications in Research

PE is an essential fluorophore for applications requiring high sensitivity, strong fluorescence intensity, and clear signal separation. It is particularly effective for:

• Detailed immunophenotyping of immune cell subsets, ensuring clear and accurate detection of specific populations in complex samples.
• Minimizing spectral overlap and enhancing clarity in multiplexed flow cytometry experiments when paired with other fluorophores like FITC, APC, and PerCP.
• Detection of rare markers and cell populations, making it indispensable for research applications requiring high sensitivity.

PE conjugated antibodies are broadly compatible with varied detection systems making them suitable for multiplexed applications such as:

• Flow cytometry and spectral flow cytometry
• Immunophenotyping
• Western blotting
• FACS
  
  

Achieve high-resolution cell sorting with multiplexed workflows

Flow cytometric analysis of mouse splenocytes, untreated (left column) or treated with cross-linked anti-CD3 plus anti-CD28 (right column), using Phospho-SLP-76 (Ser376) (E3G9U) XP^® Rabbit mAb (PE Conjugate) #76143 (top row) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (PE Conjugate) #5742 (bottom row), and co-stained with CD3 (17A2) Rat mAb (FITC Conjugate) #86603.

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use PE conjugated antibodies from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our PE Conjugated Antibodies

Need ready-made PE antibody conjugates? Explore our extensive catalog of validated products, or take advantage of our custom conjugation services and let us build the perfect tool for you.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. iFluor is a registered trademark of AAT Bioquest, Inc. iFluor is manufactured by AAT Bioquest, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}