Pacific Blue™ Antibody Conjugates

Optimize your high-dimensional flow cytometry by strategically utilizing the violet laser with Pacific Blue antibody conjugates. Pacific Blue features bright, stable blue emission and allows you to build comprehensive antibody panels without significant spectral overlap.

Flow cytometric analysis of fixed/permeabilized human peripheral blood mononuclear cells using LEF1 (C12A5) Rabbit mAb (Pacific Blue Conjugate) #57497 (right) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (Pacific Blue Conjugate) #9078 (left), co-stained with CD3 (UCHT1) Mouse mAb (redFluor 710 Conjugate) #44330.

Attributes

The dashed blue line represents the excitation spectrum of Pacific Blue and the solid line represents its emission spectrum. For multiplexing studies, antibodies with overlapping emission spectra should be avoided. Pacific Blue is optimally excited by the 405 violet laser, providing separation of its excitation and emission peaks.

Applications in Research

Researchers benefit from several distinct advantages when using Pacific Blue conjugates:

• Pacific Blue maximizes your high-parameter flow cytometry, utilizing the violet laser to allow simultaneous detection of other low-wavelength dyes.
• The brightness and stability of Pacific Blue support high-resolution imaging, capturing detailed structural information in cells and tissues with minimal photobleaching.
• Its distinct emission (~455 nm) can be easily separated from other popular fluorophores like FITC and PE, which minimizes spectral overlap when multiplexing.

Pacific Blue conjugates are suitable for use across diverse experimental setups, including:

• Flow cytometry and spectral flow cytometry
• Western blotting
• FISH

Demonstrate Specific Binding in Flow Cytometry

Flow cytometric analysis of Jurkat cells using Histone H3 (D1H2) XP^® Rabbit mAb (Pacific Blue Conjugate) #12167 (green solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (Pacific Blue Conjugate) #9078 (green dashed line) or unstained cells (orange dashed line).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Pacific Blue conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our Pacific Blue Conjugated Antibodies

Need primary or secondary Pacific Blue antibody conjugates? Explore our broad range of validated options, or take advantage of our custom conjugation services to create a solution tailored to your research.

Related or Alternative Conjugates

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Pacific Blue is a trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}