mFluor™ Red 780 Antibody Conjugates

Detect proteins and cells outside the visible spectrum with near-infrared mFluor Red 780 conjugates. mFluor Red 780 is highly photostable, reduces background noise by avoiding autofluorescence, and can be excited by standard red lasers found in flow cytometers and fluorescence microscopes (633 nm). mFluor Red 780 provides clear, reliable signals across a range of applications.

Flow cytometric analysis of fixed/permeabilized mouse splenocytes using TCF1/TCF7 (C63D9) Rabbit mAb (mFluorTM Red 780 Conjugate) #86052 (right) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (mFluorTM Red 780 Conjugate) #79088 (left).

View mFluor Red 780 Conjugated Antibodies

Attributes

The dashed red line is the absorption spectrum of mFluor Red 780, while the solid line is its emission spectrum. For optimal outcomes in multiplex labeling experiments, choosing antibodies conjugated to fluorophores with minimal emission overlap is essential. Although its excitation peaks at 633, mFluor Red 780 has a broad excitation range making it versatile with many red and near-infrared lasers.

Applications in Research

mFluor Red 780 is a powerful research tool for several reasons:

• 780 nm light is beyond the visible spectrum, giving mFluor Red 780 conjugates exceptionally low autofluorescence-induced background noise.
• In multi-color experiments, the near-infrared emission of mFluor Red 780 shares overlap with very few other fluorophores.
• mFluor Red 780 is easy to conjugate due to its variable reactive groups, water solubility, small size, and high stability. This enables efficient attachment to antibodies and biomolecules while maintaining functionality in experiments.
• Compared to APC-Alexa Fluor^® 750 tandem dyes, mFluor Red 780 is more photostable, making it readily available for fluorescence imaging applications.

mFluor Red 780 is stable and compatible with several techniques requiring fluorescent labeling, including:

• Flow Cytometry

Well-differentiated flow cytometry signals

Flow cytometric analysis of fixed/permeabilized THP-1 cells (blue, negative) and Jurkat cells (green, positive) using Helios (D8W4X) XP^® Rabbit mAb (mFluor™ Red 780 Conjugate) #72658 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (mFluor™ Red 780 Conjugate) #79088 (dashed lines).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use mFluor Red 780 conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our mFluor Red 780 Conjugated Antibodies

Are you searching for ready-to-use mFluor Red 780-conjugated primary and secondary antibodies? Check out our comprehensive catalog of validated products, or let us create a custom conjugation solution tailored to your needs.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. mFluor is a trademark of AAT Bioquest, Inc. mFluor is manufactured by AAT Bioquest, Inc. Alexa Fluor is a registered mark of Life Technologies Corporation. U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}