iFluor® 594 Antibody Conjugates
Illuminate your research with iFluor 594, a vibrant red dye compatible with fluorescence microscopy and flow cytometry systems. Its high water solubility minimizes aggregation, while reduced non-specific binding enhances signal precision. Easily conjugate iFluor 594 to various antibodies and biomolecules, enabling versatile and reliable applications.
Attributes
Excitation: 585 nm
Emission: 606 nm
Laser Line: 594 nm
Common Filter Set: 610/20
Brightness: 3
Quantum Yield: 0.66
The dashed line indicates iFluor 594's excitation and its emission spectrum is shown as the solid line. Antibodies with overlapping emission spectra should not be used together for optimal multiplexing results.
Applications in Research
iFluor 594 offers several advantages to researchers:
- iFluor 594's bright red fluorescence helps achieve high-contrast images against a tissue background.
- Its high quantum yield ensures that even low-abundance targets emit a strong, clear signal, which is critical for sensitive detection.
- Some data suggests that iFluor 594 is susceptible to photobleaching, which makes it a better option for Cyclic-IF applications than the more stable Alexa Fluor 594 dye.
- With an emission peak in the red range, iFluor 594 avoids spectral interference with commonly used fluorophores and stains such as GFP, FITC, and DAPI, making it ideal for multi-color assays.
Compatible with multiple detection systems, iFluor 594 is stable across several applications, including:
- Flow cytometry and spectral flow cytometry
- Live in vivo imaging
- Western blotting
- Immunofluorescence
- Spatial biology
- Immunohistochemistry
- Super-resolution microscopy
- FISH
Achieve clear separation during flow cytometry
Flow cytometric analysis of fixed/permeabilized MCF7 cells (blue, negative) and HeLa cells (green, positive) using Vimentin (D21H3) XP® Rabbit mAb (iFluor 594 Conjugate) #37262 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control (iFluor 594 Conjugate) #94999 (dashed lines).
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use iFluor 594 conjugates. CST uses a stringent and reproducible manufacturing process that results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our iFluor 594 Conjugated Antibodies
Searching for iFluor 594 antibody conjugates? Explore our extensive selection of validated primary and secondary conjugates, or leverage our custom conjugation services to create a tailored solution for your research needs.
Related or Alternative Fluorophores
Alexa Fluor® 594 | Texas Red | Brilliant Violet 605 |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Fluor is a registered trademark of AAT Bioquest, Inc. iFluor is manufactured by AAT Bioquest, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit our Trademark Information page.