HRP Conjugated Antibodies
Accurately detect and visualize a variety of biological targets with CST® Horseradish Peroxidase (HRP) antibody conjugates. Through the catalytic oxidation of various substrates, HRP conjugates produce a colorimetric, chemiluminescent, or fluorescent signal to identify your target while permitting signal amplification.
Western blot analysis of extracts from various cell lines using α-Actinin (D6F6) XP® Rabbit mAb (HRP Conjugate) #12413.
Attributes
High Sensitivity: HRP's robust signal amplification allows for highly sensitive detection of low-abundance targets, making it ideal for assays like Western blotting and ELISA.
Ease of use: HRP conjugates deliver rapid and strong signal development, offering simplicity and efficiency for routine lab use and high-throughput applications.
Stability: HRP conjugates are stable, dependable, and easy to store, providing cost-effective, consistent performance across multiple experiments.
Detection speed: HRP's fast enzymatic reaction enables rapid signal development, ideal for time-sensitive assays and applications.
Applications in Research
The unique enzymatic mode of action for HRP-conjugated antibodies makes them a powerful tool for research:
- HRP amplifies your output signals, enabling the detection of even low-abundance target molecules.
- HRP conjugates support colorimetric, chemiluminescent, and fluorescent detection, offering versatility for qualitative (e.g., Western blot) and quantitative (e.g., ELISA) assays.
- HRP antibody conjugates are compatible with tyramide signal amplification (TSA) to allow the detection of low-abundant targets.
- HRP-conjugated antibodies are commonly used in assays to detect biomarkers, hormones, and pathogens as well as measure protein concentrations in various biological fluids such as cell culture supernatants and serum.
HRP is broadly compatible with varied detection systems depending on the conjugation type, making it suitable for numerous applications such as:
Achieve specific and robust signal amplification
Specificity data using human IgG, IgM, and IgA at a concentration of 0.005 μg/ml. Results show no reactivity between Anti-Human IgA, alpha-Chain Specific, HRP-Linked Antibody #80403 and human IgG or IgM when used at a concentration of 1 μg/ml.
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use HRP conjugated antibodies. A stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our HRP Conjugates
Looking for an HRP conjugate to accelerate your research? Explore our extensive catalog of validated products, or let us help you design the perfect solution with our custom conjugation services.
Related or Alternative Fluorophores
Alkaline phosphatase (AP) | Soybean peroxidase (SBP) |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology, CST, XP and SignalStain are registered trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.