Fluorescein isothiocyanate (FITC) Conjugated Antibodies

Achieve reliable target detection with FITC conjugated antibodies, a widely used conjugation for labeling biomolecules. With bright green emission and excitation achieved with the standard 488 nm laser, FITC is a versatile choice for flow cytometry, immunofluorescence, and microscopy, providing strong fluorescence signals for clear, precise detection.

Confocal immunofluorescent analysis of human CD8+ T cells (left, positive) and MJ [G11] cells (right, negative) using CD8α (RPA-T8) Mouse mAb (FITC Conjugate) #55397 (green). Samples were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue).

Attributes

The excitation spectrum (dashed green line) and emission spectrum (solid green line) for FITC are shown above. With bright green emission, FITC provides a strong signal ideal for flow cytometry, immunofluorescence, and fluorescence microscopy, though it is sensitive to pH changes and photobleaching. Its broad compatibility and affordability make it a cornerstone fluorophore for many applications.

Applications in Research

FITC is a versatile fluorophore ideal for applications requiring clear green fluorescence, broad compatibility, and cost-effective labeling solutions.

• FITC is one of the most widely used dyes for antibody and protein conjugation in fluorescence applications. As a result, most instruments are equipped with a 488 nm laser and a corresponding FITC filter setup.
• FITC conjugated antibodies provide strong, distinct green signals in immunofluorescence applications, making it easy to visualize proteins or cellular structures in fixed or live-cell imaging.
• FITC is an excellent, cost-effective choice for labs needing robust and reproducible results in high-throughput assays.

FITC conjugated antibodies are broadly compatible with varied detection systems, making them suitable for multiplexed applications such as:

• Flow cytometry and spectral flow cytometry
• Immunofluorescence
• Immunophenotyping
• Fluorescence microscopy
• FISH
• Fluorescent microplate reader assays
  
  

Multiplexed tissue imaging made easy

Confocal immunofluorescent analysis of mouse spleen using CD3 (17A2) Rat mAb (FITC Conjugate) #86603 (green), Iba1/AIF-1 (E4O4W) XP^® Rabbit mAb (Alexa Fluor^® 555 Conjugate) #36618 (cyan pseudocolor), and CD45 (D3F8Q)\ Rabbit mAb (Alexa Fluor^® 647 Conjugate) #81143 (red). Sections were mounted in ProLong Gold Antifade Reagent with DAPI #8961 (blue).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use FITC conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our FITC Conjugated Antibodies

Related or Alternative Conjugates

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}