DyLight 800 Antibody Conjugates

Detect low-abundance proteins and cells with high quantum yield DyLight 800 antibody conjugates. DyLight 800 is a bright, near-infrared dye (800 nm) with a high signal-to-noise ratio and minimal crosstalk with visible spectrum dyes. It is also a photostable dye that is resistant to photobleaching and is compatible with infrared imaging systems.

Western blot analysis of Jurkat cell lysates #9194 treated with either U0126 (MEK 1/2 inhibitor) #9903 or TPA #4174, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/204) (D13.14.4E) XP® Rabbit mAb #4370 detected with Anti-rabbit IgG (H+L) (DyLight 800 4X PEG Conjugate) #5151 (green) and p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107 detected with Anti-mouse IgG (H+L) (DyLight 680 Conjugate) #5470 (red).

Attributes

The dashed line illustrates the excitation spectrum of DyLight 800, while the solid line represents its emission spectrum. In experiments with multiple dyes, best results are obtained by avoiding emission spectral overlap. DyLight 800 has a broad excitation spectrum that peaks around 777 nm but can effectively be excited by a range of NIR wavelengths, from roughly 740 nm to 800 nm.

Applications in Research

Several key advantages make DyLight 800 antibodies powerful tools for researchers:

• DyLight 800 can be used alongside another near-infrared fluorophore, such as DyLight 680, to perform dual-color Fluorescent Western blotting. This allows researchers to detect and quantify multiple proteins simultaneously on the same blot.
• It can be used to generate clearer images in thicker samples due to the high tissue penetration of near-infrared light.
• The high photostability of DyLight 800 antibodies supports accurate quantification and visualization when imaging over extended periods, making it suitable for longitudinal studies in pharmacokinetics and biodistribution.

The photostability of DyLight 800 also makes it applicable to a broad array of experiments, including:

• Fluorescent/In-cell western
• Flow cytometry and spectral flow cytometry
• Immunofluorescence
• Immunohistochemistry
  
  

Detect Signal Changes with High Sensitivity

In-Cell Western analysis of A549 cells exposed to varying concentrations of U0126 (MEK1/2 Inhibitor) #9903 for 3 hours, followed by TPA #9905 stimulation for 30 minutes. With increasing concentrations of U0126, a significant decrease (~2 fold) in Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106 signal as compared to the TPA-stimulated control was observed. Data and images were generated using Anti-mouse IgG (H+L) (DyLight 800 4X PEG Conjugate) #5257.

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use DyLight 800 conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Cy is a registered trademark of GE Healthcare. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}