Cy®5.5 Antibody Conjugates

Minimize spectral overlap and reduce auto-fluorescence with Cy^®5.5 conjugates. Positioned further into the red spectrum, Cy^®5.5 is ideal for in vivo imaging and multiplexed experiments where clear, distinct signals are critical and limited background noise from your sample material is required.

Flow cytometric analysis of 293T cells, mock transfected (blue) or transfected with a construct expressing an HA-tagged protein (green), using HA-Tag (6E2) Mouse mAb (Cy^® 5.5Conjugate) #62145 (solid lines) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (Cy^® 5.5 Conjugate) #93649 (dashed lines).

Attributes

The Cy^®5.5 excitation (red dashed line) and emission (solid red) spectra are in the near-infrared (NIR) wavelengths, making Cy^®5.5 ideal for limiting autofluorescence in samples. The spectral separation from commonly used fluorophores makes it useful in complex, multicolor panels, reducing the risk of spectral overlap.

Applications in Research

Numerous characteristics of Cy^®5.5 make it a powerful tool for research, including:

• Reduced spectral overlap and minimized auto-fluorescence make it ideal for distinguishing clear signals in multiplexed experiments and in vivo imaging.
• Can be excited by common laser lines, such as 680 nm, available on many flow cytometers and fluorescence microscopes, making it easy to integrate with existing experimental setups.

Cy^®5.5 is broadly compatible with varied detection systems and highly stable over time, making it suitable for:

• Flow cytometry and spectral flow cytometry
• Immunohistochemistry
• Immunofluorescence
• Spatial biology
• Western blotting
• FRET

Sort cells with precision using live-cell flow cytometry

Flow cytometric analysis of 293T cells transfected with a construct expressing an HA-tagged protein using HA-Tag (6E2) Mouse mAb (Cy^®5.5 Conjugate) #62145 (green solid line) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control (Cy^®5.5 Conjugate) #93649 (green dashed line) or unstained cells (orange dashed line).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Cy^®5.5 antibody conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our Cy5.5 Conjugated Antibodies

Looking for ready-made Cy^®5.5 antibody conjugates? Explore our catalog for validated products, or take advantage of our custom conjugation services and let us build the perfect tool for you.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology and CST are registered trademarks of Cell Signaling Technology, Inc. Cy and CyDye are registered trademarks of GE Healthcare. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}