Biotinylated Antibody Conjugates
Leverage the strong and specific binding of biotinylated antibodies with streptavidin/avidin to amplify weak biological signals in your research applications. CST® biotinylated antibody conjugates are an excellent option for indirect labeling experiments due to their high sensitivity and capacity for detecting low-abundance proteins or antigens with limited background interference.
Attributes
Reduced steric hindrance: Biotin’s small size minimizes conformation changes to the conjugated antibody, allowing it to retain binding affinity without disrupting the antibody-antigen interaction.
Flexible and versatile: Biotinylated antibodies can be used with various streptavidin conjugates, allowing you to use one antibody with numerous detection methods.
High sensitivity: The signal amplification provided by the biotin-streptavidin interaction is valuable for improving the reliability of assays investigating low-abundance targets.
Strong and specific binding: The biotin-streptavidin interaction is incredibly strong, ensuring biotinylated antibodies provide high specificity in detection assays.
Applications in Research
These key characteristics make biotinylated antibodies a powerful tool for research, allowing you to:
- Develop universal detection systems with biotinylated antibodies to streamline assay development.
- Build flexibility into your experimental design. For example, the same biotinylated antibody can be used with streptavidin-HRP for ELISA or streptavidin-fluorophores for flow cytometry.
- Amplify weak signals by adding several layers of biotin-streptavidin binding events. Particularly useful in assays where direct detection would result in weak or undetectable signals.
Biotinylated antibody conjugates have broad uses when coupled with streptavidin conjugates, making them suitable for numerous applications such as:
- Immunohistochemistry
- Immunoprecipitation
- ELISA and Antibody Pair-based Immunoassays
- Cell Enrichment
- Flow cytometry and spectral flow cytometry
- Western blotting
Resolve immunoprecipitation results with accuracy and precision
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) #4097. Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691.
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use Biotinylated antibody conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our Biotinylated Antibody Conjugates
Take your research to the next level with our ready-made biotinylated antibody conjugates. Explore our extensive catalog of validated products, or let us build the perfect system for you with our custom conjugation services.
Related or Alternative Conjugates
Digoxigenin (DIG) | Dinitrophenol (DNP) | Dansyl |
For Research Use Only. Not for Use in Diagnostic Procedures.
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