APC-Cy®7 Antibody Conjugates
Choose APC-Cy7 antibody conjugates to enhance multicolor flow cytometry and immunophenotyping workflows. The far-red/NIR emission (~785 nm), high brightness, and sensitivity labels low-abundance markers while minimizing background autofluorescence and spectral overlap. Easily excited by 633 or 640 nm lasers, APC-Cy®7 is ideal for panels requiring precise detection and minimal interference.
Attributes
Excitation: 650 nm
Emission: 785 nm
Laser Line: 628-640 nm
Common Filter Set: 780/60
Brightness: 2
The excitation spectrum (dashed red line) and emission spectrum (solid red) for APC-Cy®7 are shown above. This tandem fluorophore combines APC’s brightness with the Cy7 far-red emission to minimize spectral overlap and reduce background autofluorescence. Its large Stokes shift and compatibility with 633 and 640 nm laser lines make APC-Cy®7 an ideal addition for multicolor flow cytometry panels.
Applications in Research
APC-Cy®7 is invaluable in applications that require high sensitivity, clear signal separation, and minimal background interference. It is particularly effective for:
- Targeting highly expressed markers like CD4, CD8, and CD19 for detailed immunophenotyping of immune cell subsets in complex samples such as blood and tissue lysates.
- Multicolor flow cytometry panels, when paired with many of the commonly used blue, green, and orange fluorochromes like Pacific Blue™, FITC, and PE to reduce spectral overlap and compensation needs.
- Sorting specific cell populations based on surface marker expression with minimal crosstalk.
APC-Cy®7 is widely compatible with diverse detection systems, red laser lines, and filter sets, making it ideal for varied applications such as:
- Flow cytometry and spectral flow cytometry
- Immunophenotyping
- Western blotting
- FACS
Enhance the detection of low abundance targets
Flow cytometric analysis of live mouse splenocytes using CD62L/L-Selectin (MEL-14) Rat mAb (APC-Cy®7 Conjugate) #18118 (right) compared to concentration-matched Rat Isotype Control (APC-Cy®7 Conjugate) (left), co-stained with CD44 (IM7) Rat mAb (FITC Conjugate) #75122.
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use APC-Cy®7 conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our APC-Cy®7 Conjugated Antibodies
Related or Alternative Fluorophores
APC-CF 750 | mFluor Red 780 | APC-Fire 750 |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology and CST are registered trademarks of Cell Signaling Technology, Inc. Cy is a registered trademarks of GE Healthcare. Pacific Blue is a trademark of Life Technologies Corporation. mFluor is a trademark of AAT Bioquest, Inc. mFluor is manufactured by AAT Bioquest, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.