APC Conjugated Antibodies
Unlock the full potential of your immunofluorescence and flow cytometry workflows with APC conjugates. APC's far-red emission and exceptional brightness allow for the precise detection of low-abundance targets and rare cell populations. Excitable by standard 633 and 640 nm lasers, APC minimizes background autofluorescence and spectral overlap, making it the ideal choice for high-performance multicolor panels.
Attributes
Excitation (Max): 651 nm | Emission: 660 nm |
Laser Line: 628-640 nm | Common Filter Set: 660/20 |
Brightness: 5 | Quantum Yield: ~0.7 |
The excitation spectrum (dashed red line) and emission spectrum (solid red line) for APC are shown above. APC conjugates' far-red emission and high brightness minimize spectral overlap and background autofluorescence, making them ideal for multicolor flow cytometry panels. Easily excited by standard 633 or 640 nm lasers, APC conjugates ensure clear detection of low-abundance markers.
Applications in Research
APC is a powerful fluorophore offering exceptional brightness and precise detection, making it ideal for:
- Immunophenotyping in complex samples, effectively labeling cell-surface markers while ensuring intense signal clarity even in low-expression scenarios.
- Simplifying spectral compensation for multicolor flow cytometry panels by leveraging its red laser excitation and minimal overlap with fluorophores such as FITC, PE, and Alexa Fluor® 488.
- Applications demanding clear signal distinction, such as isolating specific cell populations with high sensitivity and minimal background interference, ensuring reliable and reproducible results.
Take advantage of the superior brightness of APC for numerous applications, including:
- Immunofluorescence
- Flow cytometry and spectral flow cytometry
- Immunophenotyping
- Western blotting
- FACS
Clearly detect surface markers in mixed populations
Confocal immunofluorescence analysis of fixed frozen mouse spleen, labeled with CD3 (17A2) Rat mAb (APC Conjugate) #24265 (left, red) and co-labeled with HS1 (D5A9) XP® Rabbit mAb (Rodent Specific) (Alexa Fluor® 488 Conjugate) #68206 (right, green) and DAPI #4083 (right, blue).
Commitment to Quality
Get accurate and reliable experimental results with functionally validated, ready-to-use APC conjugates. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.
Explore Our APC Conjugated Antibodies
Related or Alternative Conjugates
Alexa Fluor® 647 | Cy®5 | Brilliant Ultraviolet 661 |
For Research Use Only. Not for Use in Diagnostic Procedures.
Cell Signaling Technology and CST are trademarks or registered trademarks of Cell Signaling Technology, Inc. Cy is a registered trademark of GE Healthcare. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.