Alkaline Phosphatase Antibody Conjugates

Leverage the robust and long-lasting signal of CST^Ⓡ Alkaline Phosphatase (AP) conjugated antibodies to detect key proteins requiring prolonged signal development or long-term storage. By catalyzing phosphate removal from various substrates, AP conjugates produce a detectable signal via colorimetric, fluorescent, or chemiluminescent methods, offering high sensitivity through signal amplification.

Western blot titration of NIH-3T3 cells using p44/42 MAPK (Erk1/2) (3A7) Mouse mAb #9107 and Anti-mouse IgG, AP-linked Antibody #7056 as the secondary antibody.

Attributes

Applications in Research

Alkaline phosphatase antibody conjugates can be used with multiple detection modes for your research:

• AP catalyzes the conversion of substrates like BCIP/NBT and pNPP into a visible, often colored precipitate. These signals can be visualized directly or quantified spectrophotometrically.
• Alkaline phosphatase antibodies are commonly used with chemiluminescent substrates, such as CSPD and CDP-Star, which emit light upon reaction. This light can be detected using sensitive imaging systems.
• AP can also be used with fluorogenic substrates, expanding versatility by enabling signal detection in fluorescence-based assays.

With so many signal potentials, alkaline phosphatase-labeled antibodies can be applied in numerous experimental setups, including:

• Western blot
• ELISA
• Immunohistochemistry
• In Situ hybridization
• Immunocytochemistry
  

Visualize your target with high specificity and sensitivity

Western blot analysis of mouse IgG subclasses (10 ng per lane) reduced and denatured in 1x SDS loading buffer with DTT using Goat Anti-Mouse IgG1 Antibody (AP Conjugate) #48253 (left) or Anti-mouse IgG, AP-linked Antibody #7056 (right).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use alkaline phosphatase antibody conjugates. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our AP Conjugated Antibodies

Are you looking for validated, ready-made alkaline phosphatase antibody conjugates? Explore our catalog of readily available products, or accelerate your research with our custom conjugation services and let us build the perfect solution for you.

Related or Alternative Conjugates

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

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