Alexa Fluor® 594 Antibody Conjugates

Visualize proteins with the bright red fluorescence and efficient light absorption of Alexa Fluor 594. The longer Ex/Em wavelengths of Alexa Fluor 594 conjugates offer mild tissue penetration with minimal background interference. The excellent photostability of these conjugates allows them to withstand prolonged light exposure without photobleaching.

Confocal Immunofluorescent analysis of rat cerebellum using GFAP (GA5) Mouse mAb (Alexa Fluor^® 594 Conjugate) #8152 (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor^® 488 Conjugate) #8024 (green). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Attributes

The excitation spectrum of Alexa Fluor 594 is demonstrated as the dashed orange line while the emission spectrum is shown in solid orange. For multiplexed labeling experiments, selecting antibodies conjugated to fluorophores with limited excitation and/or emission overlap yields the best results.

Applications in Research

There are several advantages afforded by Alexa Fluor 594 conjugates that researchers can take advantage of:

• Alexa Fluor 594 can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.
• Although the emission of the Alexa Fluor 594 conjugates not in the far-red emission range, Alexa Fluor 594 conjugates benefit from their long wavelength for mild tissue penetration and reduced autofluorescence, limiting background noise from blue and green light.
• The high brightness and photostability of Alexa Fluor 594 allows the detection of low abundance molecules with high sensitivity.
• Alexa Fluor 594 is available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.

Alexa Fluor 594 is highly stable and can be used with several detection systems and is suitable for numerous multiplexed applications, including:

• Immunofluorescence
• Flow cytometry and spectral flow cytometry
• Immunohistochemistry
• Spatial biology
• Super Resolution Microscopy (SRM)

Achieve clarity when used with green and blue dyes

Immunofluorescent analysis of NTERA-2 (left) and HeLa (right) cells using Nanog (D73G4) XP^® Rabbit mAb (Alexa Fluor^® 594 Conjugate) #8750 (red). Actin filaments were labeled with Alexa Fluor^® 488 phalloidin #8878 (green).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 594 antibody conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

View Our Alexa Fluor 594 Conjugated Antibodies

Seeking Alexa Fluor^® 594 conjugated primary and secondary antibodies? Explore our broad range of validated products or opt for our custom conjugation services to craft the perfect solution for your needs.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST and XP are registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. iFluor is a registered trademark of AAT Bioquest, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}