Alexa Fluor® 532 Antibody Conjugates

Tag your proteins or cells of interest with the bright, highly photostable yellow fluorescence of Alexa Fluor 532. Distinct from green and red fluorophores, Alexa Fluor 532 conjugates are useful in multicolor applications, including flow cytometry and super-resolution microscopy using dSTORM. The common 532 green laser line and 555/20 filter set can be used with Alexa Fluor 532 conjugates for seamless integration into your current workflows.

Flow cytometric analysis of fixed/permeabilized mouse splenocytes using TCF1/TCF7 (C63D9) Rabbit mAb (Alexa Fluor^Ⓡ 532 Conjugate) #46382 (right) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (Alexa Fluor 532 Conjugate) #42387 (left).

Attributes

The dashed green line represents the excitation spectrum of Alexa Fluor 532, and the solid line shows its emission spectrum. For optimal results in multiplex labeling experiments, it’s best to choose antibodies conjugated to fluorophores with minimal overlap in emission spectra. Alexa Fluor 532 conjugates can be excited in the 515-532 nm range.

Applications in Research

Alexa Fluor 532 is a powerful tool for research for several reasons:

• It is useful in multiplexing experiments as it has little overlap with red, green, blue, far-red, and near-infrared fluorophores, including Alexa Fluor® 488.
• Alexa Fluor 532 conjugates are commonly used in super-resolution microscopy, where their photostability and brightness enable the capture of detailed images.
• Alexa Fluor 532 is particularly popular in flow cytometry due to its optimal excitation with the 532 nm laser and its bright, sharp emission.
• It is available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.

Alexa Fluor 532 is compatible with several detection systems and is pH insensitive over a wide molar range, ensuring stable signals in live cells and in:

• Flow cytometry and spectral flow cytometry
• Immunofluorescence
• FRET
• Super-resolution microscopy

Well-differentiated flow cytometry signals

Flow cytometric analysis of HeLa cells using Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Alexa Fluor 532 Conjugate) #54575 (green solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (Alexa Fluor^® 532 Conjugate) #42387 (green dashed line) or unstained cells (orange dashed line).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 532 conjugates from CST. Our stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our Alexa Fluor 532 Conjugated Antibodies

Looking for Alexa Fluor 532-conjugated primary and secondary antibodies? Browse our wide selection of validated products, or use our custom conjugation services to create the ideal solution for your research needs.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. iFluor is a registered trademark of AAT Bioquest, Inc. iFluor is manufactured by AAT Bioquest, Inc. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}