Alexa Fluor® 488 Antibody Conjugates

Illuminate complex cellular processes and components with precision using Alexa Fluor 488 antibody conjugates. This green fluorescent dye offers a photostable solution with enhanced brightness and hydrophilicity and can be conjugated to proteins and modified oligos for multiplexed biological analyses.

Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) XP^® Rabbit mAb (Alexa Fluor^® 488 Conjugate) #9854 (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Attributes

The excitation spectrum for Alexa Fluor 488 is represented by the dashed green line, with the emission spectrum shown in solid green. For multiplexed labeling experiments, selecting antibodies conjugated to fluorophores with limited emission overlap yields the best results.

Applications in Research

Several key characteristics of Alexa Fluor 488 make it a powerful tool for research:

• Alexa Fluor 488 provides superior photostability, brightness, and conjugate stability over FITC while providing limited spectral overlap with common fluorophores.
• Hydrophilic properties make it suitable for conjugation with sensitive proteins to maintain solubility and limit aggregate formation in solution.
• Available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.

Alexa Fluor 488 is broadly compatible with varied detection systems and highly stable over a range of pH, making it suitable for multiplexed applications such as:

• Immunohistochemistry
• Immunofluorescence
• Flow cytometry and spectral flow cytometry
• Spatial biology
• FRET
• FISH

Perform flow cytometry with high precision

Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D2C8) XP^® Rabbit mAb (Alexa Fluor^® 488 Conjugate) #3465 compared to propidium iodide (DNA content). The box indicates phospho-histone H3 positive cells.

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 488 conjugates. CST uses a stringent and reproducible manufacturing process resulting in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our Alexa Fluor 488 Conjugated Antibodies

Do you need ready-made Alexa Fluor 488 secondary antibody and primary antibody conjugates? Explore our extensive catalog of validated products, or take advantage of our custom conjugation services and let us build the perfect tool for you.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST, and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}