Alexa Fluor® 350 Conjugated Antibodies

Differentiate your molecular targets from other fluorophores using the intense blue emission of Alexa Fluor 350 antibody conjugates. Alexa Fluor 350 can be excited in the ultraviolet spectrum, is photostable, avoids most blue-green autofluorescence, and is ideal for high-abundance targets.

Flow cytometric analysis of fixed/permeabilized MCF7 cells (blue, negative) and SNB-19 cells (green, positive) using Vimentin (D21H3) XP^® Rabbit mAb (Alexa Fluor^® 350 Conjugate) #62006 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (Alexa Fluor® 350 Conjugate) #34051 (dashed lines).

Attributes

The excitation spectrum (dashed blue line) and emission spectrum (solid blue) of Alexa Fluor 350 are shown above. Selecting fluorophores with minimal excitation and emission spectral overlap yields the best results when multiplexing. Alexa Fluor 350 has a broad excitation range, allowing it to be excited by ultraviolet (330 nm) and conventional violet lasers (355 nm).

Applications in Research

There are many characteristics of Alexa Fluor 350 that can be leveraged for maximum impact in your research:

• The deep blue of Alexa Fluor 350 is ideal for tagging background or abundant proteins or cells for multiplexed flow cytometry with the bright green of Alexa Fluor^® 488.
• Available with various reactive groups to allow conjugation with most biomolecules, permitting access to amine, thiol, glycan, or other conjugation chemistries.

Alexa Fluor 350 has broad compatibility with several detection systems and is suitable for numerous multiplexed applications, including:

• Flow Cytometry
• Immunofluorescence
• Western blotting
• FRET
• Super-resolution microscopy

High performance in flow cytometry

Flow cytometric analysis of fixed/permeabilized 293T cells, mock transfected (blue) or transfected with a construct expressing an HA-tagged protein (green), using HA-Tag (C29F4) Rabbit mAb (Alexa Fluor^® 350 Conjugate) #34888 (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control (Alexa Fluor^® 350 Conjugate) #34051 (dashed lines).

Commitment to Quality

Get accurate and reliable experimental results with functionally validated, ready-to-use Alexa Fluor 350 conjugates from CST. Stringent and reproducible manufacturing process results in high-quality antibody conjugates produced to the same standards you've trusted for over 25 years. For custom or made-to-order conjugates, we rely on our decades of experience, thorough biophysical characterization, and parent antibody validation to deliver a product with high specificity and sensitivity. Every order comes with comprehensive product documentation and access to expert technical support.

Explore Our Alexa Fluor 350 Conjugated Antibodies

Looking for Alexa Fluor 350-conjugated primary antibodies? Browse our range of validated products, or use our custom conjugation services to create the perfect solution tailored to your needs.

Related or Alternative Fluorophores

_{For Research Use Only. Not for Use in Diagnostic Procedures.}

_{Cell Signaling Technology, CST and XP are trademarks or registered trademarks of Cell Signaling Technology, Inc. Alexa Fluor is a registered trademark of Life Technologies Corporation. All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.}