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Loading Control Antibodies

Loading control antibodies are used to determine equal loading as well as the quality of the samples. This is critically important to aid in the interpretation of western blot results. Proteins used as loading controls are typically selected for their ubiquitous expression across all cell lines and tissues as well as their abundance. Selecting the right loading control antibody is determined by the molecular weight of the other targets being studied, subcellular fraction of the sample, and the cell and tissue types being evaluated.

You need to trust that your loading control is sensitive and specific to its target protein. If your loading control is questionable, it can create doubt about all of your results. Use loading controls you trust for the most reliable experimental results.

Loading Control Antibodies

Loading ControlSize (kDa)Recommended for:
β-Actin45Whole Cell Lysate
GAPDH37
β-Tubulin55
α-Tubulin52
Vinculin124
α-Actinin100
Cyclophilin B19
Loading ControlSize (kDa)Recommended for:
Histone H317Nuclear Fraction
HDAC162
Loading ControlSize (kDa)Recommended for:
COX IV17Mitochondrial Fraction
VDAC32
SDHA70

Loading Control Antibody Sampler Kits

No.

Name

5142Loading Control Antibody Sampler Kit
9774Loading Control Antibody Sampler Kit (Mouse)
4670Loading Control Antibody Sampler Kit (HRP Conjugate)