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ChIP-seq Ab Performance Comparison

Choosing an antibody that recognizes the correct target protein and does not show non-specific binding with other DNA-binding proteins is critical for a successful ChIP-seq experiment. CST now tests and validates recombinant rabbit monoclonal antibodies in ChIP-seq, because good performance in ChIP-qPCR does not necessarily mean an antibody will perform well for ChIP-seq, which requires more extensive capture of the target protein across a large number of gene loci. Our ChIP-seq approved antibodies are tested using the SimpleChIP Enzymatic Chromatin IP protocol followed by next-generation sequencing (NGS).

Advantages of using ChIP-seq antibodies produced at CST are:

  • All ChIP-seq approved antibodies from CST are recombinant rabbit monoclonal antibodies, providing greater lot-to-lot reproducibility
  • Antibodies are validated and optimized using the SimpleChIP Enzymatic Chromatin IP protocol, saving you time and money
  • Antibodies are tested and validated for target specificity across multiple applications, providing reduced non-specific binding and high signal-to-noise ratio in ChIP-seq
  • Antibodies are compatible with other ChIP-seq protocols, allowing for use in ChIP kits from CST or other suppliers, and in customized lab protocols
  • Expert technical support is available from the same scientists who developed and validated the ChIP-seq antibodies

CST has compared many of our ChIP-seq antibodies to ChIP-seq approved antibodies from other companies. Click on any of the CST antibodies below to see how we perform against the competition.

  • NEBNext is a registered trademark of New England Biolabs, Inc.
  • Ultra is a trademark of New England Biolabs, Inc.
  • Illumina is a registered trademark of Illumina, Inc.

BRD4 (E2A7X) Rabbit mAb #13440

CTCF (D31H2) XP® Rabbit mAb #3418

Ezh2 (D2C9) XP® Rabbit mAb #5246

Mono-Methyl-Histone H3 (Lys4) (D1A9) XP® Rabbit mAb #5326

Phospho-Rpb1 CTD (Ser2) (E1Z3G) Rabbit mAb #13499

Phospho-Rpb1 CTD (Ser5) (D9N5I) Rabbit mAb #13523

Rpb1 NTD (D8L4Y) Rabbit mAb #14958

Tri-Methyl-Histone H3 (Lys9) (D4W1U) Rabbit mAb #13969

Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb #9733