Cell Signaling Technology Logo - Extra Large

CXCL10 (F9N8I) & CO-0235-647 SignalStar® Oligo-Antibody Pair #93740

Filter:
  • IHC

Order Information # 93740

This product is not sold separately. Please see the SignalStar® Multiplex IHC Panel Builder Tool for ordering information.

    Product Information

    Product Usage Information

    Application Dilution
    SignalStar™ Leica Bond 1:50 - 1:200
    SignalStar™ Manual 1:50 - 1:200

    Storage

    SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

    Product Description

    SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

    SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

    Protocol

    Specificity / Sensitivity

    CXCL10 (F9N8I) Rabbit Monoclonal Antibody (SignalStar® Conjugate 0235) recognizes endogenous levels of total CXCL10 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CXCL10 protein.

    Background

    C-X-C motif chemokine ligand 10 (CXCL10, IP-10) is a chemokine produced by several cell types and induced in response to pro-inflammatory cytokines, such as IFN-gamma and TNF-alpha (1,2). CXCL10 binds to the chemokine receptor CXCR3 and induces calcium flux and cell migration (3). The CXCR3 protein is normally expressed on activated CD4+ T cells and NK cells, which can produce pro-inflammatory cytokines, including IFN-gamma and TNF-alpha (3,4). As a result, these molecules act in an amplification loop to promote immune responses in the contexts of both autoimmunity and a tumor immune response (5,6).
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    SignalStar is a registered trademark of Cell Signaling Technology, Inc.
    U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.