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  3. Streptavidin (APC Conjugate)

Streptavidin (APC Conjugate) #15859

    Product Information

    Product Description

    Streptavidin is conjugated to allophycocyanin (APC) fluorescent dye under optimal conditions and is useful for detecting biotinylated proteins (1,2).

    Fluorescent Properties

    • ← Excitation: 652 nm ← Emission: 658 nm

    Storage

    Supplied in a lyophilized form that is stable for 12 months after receipt. Upon receipt, store at -20°C. Reconstitute with 1 mL of ddH2O and store at 4°C. Upon reconstitution, the product is in 10 mM tris (pH 8.2), 150 mM NaCl, 50 mM sucrose, 1 μg/mL pentachlorophenol, and 0.1% BSA. Use resuspended material within three months. If further dilution of the conjugate is required, use within one week.

    Protect from light. Avoid multiple freeze/thaw cycles.

    Specificity / Sensitivity

    Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

    Source / Purification

    Streptavidin is a protein that is derived from the bacteria Streptomyces avidinii. Streptavidin (APC Conjugate) is purified by SEC-HPLC.

    Background

    Streptavidin is a 53 kDa homotetramer isolated from Streptomyces avidinii for use in isolation and detection bioassays (3). Each streptavidin subunit forms high affinity non-covalent bonds with the vitamin biotin. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate and detect biotinylated proteins (1,2).

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
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