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  3. Goat Anti-Rabbit IgG, Fc Fragment Antibody (Alexa Fluor® 594 Conjugate)

Goat Anti-Rabbit IgG, Fc Fragment Antibody (Alexa Fluor® 594 Conjugate) #70872

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  • IF

    Product Specifications

    REACTIVITY Rab
    SENSITIVITY
    MW (kDa)
    Source/Isotype Goat
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • Rab-Rabbit 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 1.5 mg/mL.

    Product Usage Information

    The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:500 – 1:2,000 should yield acceptable results for immunofluorescent assays.

    When using rabbit Fc-directed secondaries, a higher concentration of primary antibody may be required. The optimal dilution of each primary antibody should be determined by titration.

    Storage

    Supplied lyophilized with a formulation of 0.01 M sodium phosphate, 0.25 M sodium chloride (pH 7.6), 15 mg/mL BSA, and 0.05% azide at 4ºC. If not clear, rehydrate with 0.75 mL dH2O and centrifuge. The product is stable for six weeks at 4ºC as an undiluted liquid. For extended storage, aliquot and freeze at -80ºC, or add an equal volume of glycerol (ACS grade or better) to a final concentration of 50% and store at -20ºC. Solutions should be used within 12 months. Aliquot to avoid multiple freeze/thaw cycles. Protect from light.

    Specificity / Sensitivity

    Goat Anti-Rabbit IgG, Fc Fragment Antibody (Alexa Fluor® 594 Conjugate) reacts with the Fc portion of Rabbit IgG heavy chain, but does not cross-react with the Fab portion of rabbit immunoglobulins. This antibody conjugate also has minimal cross-reaction with human serum proteins and may cross-react with immunoglobulins from other species; however, it does not react with non-immunoglobulin serum proteins.

    Species Reactivity:

    Rabbit

    Background

    This product is optimized for use as a secondary antibody in immunofluorescent applications. Fluorescent anti-species IgG conjugates are ideal for immunofluorescence. Cell Signaling Technology applies strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.

      Limited Uses

      Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

      Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

      For Research Use Only. Not for Use in Diagnostic Procedures.
      Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
      Alexa Fluor is a registered trademark of Life Technologies Corporation.
      This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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