Cas9 (S. pyogenes) (6xHis-Tag) Recombinant Protein Quantitative Control #47140
- ELISA
Product Specifications
| MW (kDa) | 161 |
Application Key:
- ELISA-ELISA
Product Information
Product Usage Information
Prior to use, thaw the recombinant protein and briefly spin down to collect all liquid at the bottom of the tube. Dilute according to the following instructions depending on the ELISA kit type. An example sensitivity curve is shown in Figure 2.
PathScan® ELISA Kits:
Dilute with ELISA Sample Diluent to achieve a 1X protein concentration.
PathScan® RP ELISA Kits:
Dilute with 1X Cell Lysis Buffer #9803 to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the detection antibody.
FastScan™ ELISA Kits:
Dilute with 1X FastScan™ ELISA Cell Extraction Buffer to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the antibody cocktail.
Formulation
Expression Host: E. coli
Supplied in 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) glycerol.
Storage
Product Description
Human Cas9 (S. pyogenes) (6xHis-Tag) Recombinant Protein Quantitative Control is derived from full-length Cas9 (S. pyogenes) with a nuclear localization signal (NLS) and 6xHis-Tag on the carboxy-terminal end. The protein can be used with FastScan™ Cas9 (S. pyogenes) ELISA Kit #29666 and Cas9 (S. pyogenes) Matched Antibody Pair #19707 as a quantitative control. Suitable for use in other Cas9 (S. pyogenes) assays when a control is needed.
| MW (kDa) | 161 |
| Purity | Assessed to be >95% by densitometry using SDS-PAGE analysis and Coomassie Blue detection. |
Background
- Horvath, P. and Barrangou, R. (2010) Science 327, 167-70.
- Wiedenheft, B. et al. (2012) Nature 482, 331-8.
- Singh, P. et al. (2015) Genetics 199, 1-15.
- Cong, L. et al. (2013) Science 339, 819-23.
- Mali, P. et al. (2013) Science 339, 823-6.
- Li, D. et al. (2013) Nat Biotechnol 31, 681-3.
- Shen, B. et al. (2013) Cell Res 23, 720-3.
- Niu, Y. et al. (2014) Cell 156, 836-43.
Limited Uses
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