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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

UBE3C (F2S2J) Rabbit mAb #32920

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  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 123
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:50 - 1:200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    UBE3C (F2S2J) Rabbit mAb recognizes endogenous levels of total UBE3C protein. Reactivity for immunofluorescence is human only.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly660 of human UBE3C protein.

    Background

    Ubiquitin-Protein Ligase E3C (UBE3C) is an E3 ubiquitin-protein ligase that contributes to the regulation of multiple cellular processes through ubiquitination of various protein substrates, targeting them for proteasomal degradation. UBE3C activates the Wnt/β-catenin pathway, promoting proliferation and inhibiting apoptosis in cancer cells (1,2). UBE3C-mediated protein degradation also regulates autophagy signaling (3,4), viral replication (5), type I interferon signaling (6), and radioresistance (7). In addition, UBE3C participates in eliminating misfolded protein aggregates by targeting misfolded proteins for degradation (8).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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