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Primary Antibodies /
Tid-1 (RS13) Mouse mAb

Tid-1 (RS13) Mouse mAb #4775

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Supporting Data

REACTIVITY	H M R
SENSITIVITY	Endogenous
MW (kDa)	37 Tid-1s. 40 Tid-1L.
Source/Isotype	Mouse IgG1

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human
M-Mouse
R-Rat

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

Tid-1 (RS13) Mouse Monoclonal Antibody detects endogenous levels of the short and long variants of Tid-1.

Species Reactivity:

Human, Mouse, Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human Tid-1 protein. Antibody is supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 mg/ml BSA and 50% glycerol.

Background

Human Tid-1 is a human orthologue of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l (2) tid and is a member of the DnaJ family of proteins that serve as co-chaperones to Hsp70 proteins (1). These proteins are characterized by a J domain, a highly conserved tetrahelical domain that binds to Hsp70 chaperones and activates their ATPase activity. Hsp70 and their associated chaperones mediate a variety of activities including the folding of newly synthesized polypeptides, the translocation of proteins across membranes and assembly of multimeric protein complexes. Two alternatively spliced variants exist for human Tid-1 ,designated hTID-1s and hTID-1L, both which contain the J domain, localize to the mitochondrial matrix, and co-immunoprecipitate with Hsp70. Expression of Tid-1L increases apoptosis induced by the DNA damaging agent mitomycin c (MMC) and by TNF-alpha, and that activity is dependent on its J domain. In contrast, expression of Tid-1S reduces apoptosis by these agents. Tid-1 orthologues are also found in mouse (mTid-1) and rat (rTid-1) (2,3). The mouse orthologue was originally identified though its interaction with p120 GTPase-activating protein (GAP), raising the possiblity that Tid-1 helps regulates the confirmation, activity, or subcellular localization of GAP (3).

Alternate Names

hTid-1; mTid-1; rTid-1; TID1

Database Links

UniProt ID: Q96EY1

Entrez-Gene Id: 9093

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