Cell Signaling Technology Logo
1% for the planet logo
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

TERT (F3C6F) Rabbit mAb #89040

Filter:
  • WB

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 125
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    TERT (F3C6F) Rabbit mAb recognizes endogenous levels of total TERT protein. This antibody specifically detects TERT isoform 1 (O14746-1) and TERT isoform 3 (O14746-3) proteins and an 85 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TERT protein.

    Background

    Telomerase reverse transcriptase (TERT) is a key component of telomerase (1), which is a ribonucleoprotein (RNP) complex responsible for extending telomere DNA to maintain telomere length and avoid cell death. TERT expression is often very low in normal cells, with the exception of those that require continuous proliferation (2). Increased telomerase is universal in human cancers (3). In cancer, TERT activity is mainly regulated by transcriptional activation through promoter hotspot mutations (4). Gene amplification through chromosomal rearrangement and promoter methylation also play roles in upregulating TERT (5). Germline reduction in telomerase precipitates tissue-failure diseases characterized by very short telomeres, such as aplastic anemia, dyskeratosis congenita, and pulmonary fibrosis (6).
    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.