Cell Signaling Technology Logo - Extra Large
View Featured Offers >>
1% for the planet logo
  1. Products /
  2. Primary Antibodies /
  3. S-Tag (D2K2V) XP® Mouse Chimeric mAb
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

S-Tag (D2K2V) XP® Mouse Chimeric mAb #59352

Filter:
  • IF

    Product Specifications

    REACTIVITY All
    SENSITIVITY Transfected Only
    MW (kDa)
    Source/Isotype Mouse chimeric IgG2a
    Application Key:
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • All-All Species Expected 

    Product Information

    Product Description

    This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as S-Tag (D2K2V) XP® Rabbit mAb #12774.

    Product Usage Information

    Application Dilution
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    S-Tag (D2K2V) XP® Mouse Chimeric mAb recognizes transfected levels of recombinant protein containing an S-Tag. This antibody detects exogenously expressed S-tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.

    Species Reactivity:

    All Species Expected

    Source / Purification

    This recombinant chimeric antibody is engineered from S-Tag (D2K2V) XP® Rabbit mAb #12774 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a mouse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

    The parent antibody, S-Tag (D2K2V) XP® Rabbit mAb #12774, is produced by immunizing animals with a synthetic S-Tag peptide.

    Background

    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

    S-Tag is an epitope tag composed of a 15 residue peptide that, together with S-protein, comprises pancreatic ribonuclease S (1).

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    XP is a registered trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.