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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

PPP1R17 (F3O5C) Rabbit mAb #40165

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  • WB
  • IP
  • IHC
  • IF

    Supporting Data

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunohistochemistry (Paraffin) 1:800 - 1:3200
    Immunofluorescence (Frozen) 1:6400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    PPP1R17 (F3O5C) Rabbit mAb recognizes endogenous levels of total PPP1R17 protein. Staining of uncertain specificity was observed in adipose tissue, intercalated discs of the heart, and kidney proximal tubules by immunohistochemistry.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse PPP1R17 protein.

    Background

    Protein phosphatase 1 regulatory subunit 17 (PPP1R17) inhibits phosphatase activities of protein phosphatase type 1 (PP1) and protein phosphatase type 2A (PP2A) complexes (1). Its expression in the cerebellum is restricted to Purkinje cells, where it can be used as a marker for this cerebellar cell type (2). PPP1R17 expression has also been found in a subpopulation of neurons in the dorsomedial hypothalamus (DMH) that regulate restriction of food intake, aging, and longevity in mice (3,4).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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