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  3. Phospho-Neurofilament-H Motif [(A/V)KsP(V/A/E)] (F7G5E) Rabbit mAb
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Phospho-Neurofilament-H Motif [(A/V)KsP(V/A/E)] (F7G5E) Rabbit mAb #27211

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  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 180-220
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:200 - 1:800

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Phospho-Neurofilament-H Motif [(A/V)KsP(V/A/E)] (F7G5E) Rabbit mAb recognizes endogenous levels of Neurofilament-H protein only phosphorylated at Ser residues surrounded by Ala or Val at the -2 position, Lys at the -1 position, Pro at the +1 position, and Val, Ala, or Glu at the +2 position. This antibody recognizes the VKsPA motif most sensitively. Non-specific labeling of thymus and testis is observed by immunofluorescence in fixed frozen mouse tissue.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic phospho-Ser Neurofilament-H motif (A/V)KsP(V/A/E) peptide.

    Background

    The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders, including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

    Database Links

    UniProt ID: P12036


    Entrez-Gene Id: 4744


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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