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  3. OAT (F5L4R) Rabbit mAb
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Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

OAT (F5L4R) Rabbit mAb #66518

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  • WB
  • IF
  • F

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:400 - 1:1600
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    OAT (F5L4R) Rabbit mAb recognizes endogenous levels of total OAT protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala52 of human OAT protein.

    Background

    Mitochondrial ornithine aminotransferase (OAT) catalyzes the reversible conversion of L-ornithine and 2-oxoglutarate to L-glutamate 5-semialdehyde and L-glutamate (1). Studies show that pancreatic ductal adenocarcinoma (PDA) relies on de novo ornithine synthesis from glutamine, a process mediated by OAT. Ornithine derived from glutamine is used for polyamine synthesis and is critical for PDA growth. In contrast, most adult normal tissues are dependent on ornithine derived from arginine for polyamine synthesis (2). In addition, it was shown that soluble dietary fiber remodels gut microbiota composition and enhances the abundance of Bacteroides acidifaciens, which promotes OAT expression in hepatocytes. OAT facilitates ammonia detoxification in the liver and, therefore, reduces alcohol-induced liver injury in mice (3).

    Database Links

    UniProt ID: P04181


    Entrez-Gene Id: 4942


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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