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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

NPAT (F1D9Q) Rabbit mAb #76072

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  • WB
  • IP
  • IF

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 210
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50
    Immunoprecipitation 1:50
    Immunofluorescence (Immunocytochemistry) 1:3200

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    NPAT (F1D9Q) Rabbit mAb recognizes endogenous levels of total NPAT protein. This antibody detects a 120 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the central region of human NPAT protein.

    Background

    Nuclear protein of the ataxia-telangiectasia locus (NPAT) is a transcriptional coactivator that is crucial for cell cycle progression (1,2). NPAT expression is regulated by the E2F transcription factor and its protein levels peak at the G1/S boundary (2,3). NPAT regulates the expression of all five histone gene subtypes (H1, H2A, H2B, H3, and H4) during the transition from G1 to S phase of the cell cycle (4,5). NPAT serves as a scaffolding protein for the formation of histone locus bodies (HLBs), which are unique biomolecular condensates functioning as localized hubs for histone mRNA synthesis and processing (6). Phosphorylation of NPAT by cyclin-dependent kinase 2 (CDK2) is required for histone mRNA transcription, but it is not necessary for HLB formation (7).  Mutations in the NPAT gene have been linked to various cancers, including colorectal cancer and Hodgkin lymphoma (8,9). 
    For Research Use Only. Not for Use in Diagnostic Procedures.
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