Cell Signaling Technology Logo - Extra Large
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MHC Class II (I-A beta) (F7M6G) Rabbit Monoclonal Antibody #76874

Filter:
  • WB

    Product Specifications

    REACTIVITY M R
    SENSITIVITY Endogenous
    MW (kDa) 28
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MHC Class II (I-A beta) (F7M6G) Rabbit Monoclonal Antibody recognizes endogenous levels of total MHC Class II (I-A beta) protein. This antibody does not cross-react with MHC Class II (I-E beta) protein.

    Species Reactivity:

    Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln252 of mouse MHC Class II (I-A beta) protein.

    Background

    Major histocompatibility complex class II (MHC class II) molecules are heterodimeric, transmembrane glycoproteins expressed on the surface of antigen-presenting cells, such as macrophages, dendritic cells, and B cells. Expression can also be induced on other cell types through interferon-γ signaling (1). Prior to being displayed on the cell membrane, MHC class II molecules are loaded with exogenous peptide antigens approximately 15-24 amino acids in length that were derived from endocytosed extracellular proteins digested in the lysosome (2). Antigen-presentation through MHC class II is required for T cell activation during the immune response to extracellular pathogens (2). In humans, the MHC class II protein complex is encoded by the human leukocyte antigen gene complex (HLA). HLAs corresponding to MHC class II are HLA-DP, HLA-DM, HLA-DOA, HLA-DOB, HLA-DQ, and HLA-DR (3).
    In mice, MHC class II molecules are encoded by the H2 gene complex, which includes two main isotypes: I-A and I-E. I-A and I-E are each composed of an α and β chain. The expression and polymorphism of these molecules vary between mouse strains, influencing immune responses, disease susceptibility, and compatibility in transplantation models (4).

    Alternate Names

    Abe; Abeta; AI845868; H-2 class II histocompatibility antigen, A beta chain; H-2 class II, A beta; H-2A; H-2Ab; H2-A; H2-Ab; H2-Ab_; H2-Ab1; H2-iabeta; HB2A; histocompatibility 2, class II antigen A, beta 1; histocompatibility 2, class II antigen A, beta, promoter 1; I-A<; I-Ab; I-Abeta; Ia-2; Ia2; IAb; major histocompatibility complex class II beta chain; MHC class II H2-IA-beta-psi; response to metastatic cancers 1; Rmc; Rmcs1

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.