Cell Signaling Technology Logo - Extra Large
View Featured Offers >>
  1. Products /
  2. Primary Antibodies /
  3. MDC1 (F6C8T) Rabbit Monoclonal Antibody
Trial Size Available Flag
Recombinant Flag
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

MDC1 (F6C8T) Rabbit Monoclonal Antibody #79210

Filter:
  • WB

    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 250-300
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    MDC1 (F6C8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total MDC1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of MDC1. This antibody detects an approximately 100 kDa protein of unknown identity in some cell lines.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro2076 of human MDC1 protein.

    Background

    Mediator of DNA damage checkpoint protein 1 (MDC1) is a scaffold protein that plays a central role in the early stages of the DNA damage response (DDR) and helps to maintain genomic stability (1). It is among the first proteins to accumulate at sites of DNA double-strand breaks (DSBs), where it forms a complex with phosphorylated histone H2AX (γ-H2AX), protecting its C-terminal tail from premature dephosphorylation until DSB repair is complete (1,2). Through phosphorylation-dependent interactions, MDC1 recruits DNA damage responsive E3 ubiquitin ligase RNF8, which ubiquitinates histones H2A and H2AX, leading to the accumulation of repair proteins, including 53BP1 and BRCA1, at the damage site (2-5). MDC1 also recruits E3 ubiquitin ligases Pellino-1 and -2 to DNA DSB sites and is essential for DNA damage-induced cell cycle arrest at the S and G2/M checkpoints (4-6).

    Due to its critical role in the DDR, MDC1 expression levels are being studied in relation to various cancers. Studies have shown that MDC1 may function as a tumor suppressor and MDC1 loss or reduction correlates with later-stage tumors, highlighting its potential as a biomarker for prognosis and therapeutic response (1,5).

    Alternate Names

    DKFZp781A0122; homologue to Drosophila photoreceptor protein calphotin; KIAA0170; MDC1; mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; MGC166888; NFBD1; Nuclear factor with BRCT domains 1

    Database Links

    UniProt ID: Q14676


    Entrez-Gene Id: 9656


    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not for Use in Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.