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LDHA (F3T9U) Rabbit Monoclonal Antibody #71368

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  • F

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 37
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunohistochemistry (Paraffin) 1:400 - 1:1600
    Immunofluorescence (Frozen) 1:400
    Immunofluorescence (Immunocytochemistry) 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    LDHA (F3T9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total LDHA protein. This antibody does not cross-react with LDHB or LDHC protein.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse LDHA protein.

    Background

    Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.

    Alternate Names

    L-lactate dehydrogenase A chain; l7R2; lactate dehydrogenase 1, A chain; lactate dehydrogenase A; lactate dehydrogenase A4; lactate dehydrogenase-A; LDH; LDH muscle subunit; Ldh-; Ldh-1; LDH-A; LDH-M; Ldh1; Ldha; Ldhm; M-LDH; OTTMUSP00000017774

    For Research Use Only. Not for Use in Diagnostic Procedures.
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