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  3. HP1 Family Antibody Sampler Kit

HP1 Family Antibody Sampler Kit #94456

    Product Information

    Kit Usage Information

    Protocols

    Product Description

    The HP1 Family Antibody Sampler Kit provides an economical means of detecting HP1 proteins. The kit includes enough antibodies to perform two western blot experiments with each primary antibody.

    Background

    Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher-order chromatin structure (1). All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome (2,3). HP1 proteins are approximately 25 kDa and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin (4,5). The variable hinge region binds both RNA and DNA in a sequence-independent manner (6). The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin assembly factor 1 (CAF-1) (7-9). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H1 are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells (10,11). HP1 proteins are subject to multiple post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting various means of regulation (12-14).

    Database Links

    UniProt ID: Q13185 , P45973 , P83916


    Entrez-Gene Id: 11335 , 23468 , 10951


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