HMCES (F4E4Q) Rabbit mAb #65616
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Background
During somatic hypermutation (SHM) in B cells, HMCES suppresses deletions within immunoglobulin (Ig) genes but allows other types of point mutations to occur, resulting in antigen-specific high-affinity antibodies (4). HMCES deficiency impairs class switch recombination (CSR) in B cells, leading to weakened antibody production (5).
In APOBEC3A-expressing tumors, disruption of HMCES may increase the tumor’s sensitivity to therapies such as ionizing radiation (IR), oxidative stress, and ATR inhibition (6).
- Amidon, K.M. and Eichman, B.F. (2020) DNA Repair (Amst) 94, 102903.
- Mohni, K.N. et al. (2019) Cell 176, 144-153.e13.
- Rua-Fernandez, J. et al. (2023) Cell Rep 42, 113427.
- Wu, L. et al. (2022) Genes Dev 36, 433-450.
- Shukla, V. et al. (2020) Mol Cell 77, 384-394.e4.
- Biayna, J. et al. (2021) PLoS Biol 19, e3001176.
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